FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562539
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5970.1061259752657149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20150.040.9115371
TATCAAC23550.034.406272
CAACGCA24500.033.072155
ATCAACG24450.032.999363
TCAACGC24350.032.9938854
AACGCAG25300.032.1620986
CGTGCAA901.7175807E-731.18947857
GGTATCA11300.030.7010021
ACGCAGA26750.030.418737
AGAGTAC27350.030.1306911
CGCAGAG27450.029.6430268
CAGAGTA28100.029.20429410
AAACGGG605.39701E-428.76970338
GTGGTAT3250.028.5357951
GCAGAGT28700.028.4741139
GCGGCAT900.007612967427.68374370
GAGTACG22200.027.06700712
AGTACGG22100.027.03411113
TACGGGG9500.026.7486515
AATAGCG1109.395044E-725.68197662
Produced by FastQC (version 0.11.5)