FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562539
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10370.18434277445652658No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6350.11288106246855774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC200.006502464453.39120565
GTATCAA21400.037.362651
TATCAAC23750.033.5212252
ATCAACG24200.032.7560963
TCAACGC24400.032.6282424
CAACGCA24550.032.5686655
AACGCAG24850.031.9021346
ATCGTCT553.0700772E-431.67778649
ACGCAGA26050.030.4352597
CGCAGAG26750.029.6440938
GGTATCA12800.029.4902361
AGAGTAC26950.029.424111
GCTTCGG605.576513E-428.60930819
CAGAGTA28100.028.2199110
GCAGAGT28550.027.7751129
CGTAGCA500.00792235127.46249615
AGTACGG21800.027.24203913
GAGTACG21950.026.8994812
GCTAGCC658.305292E-426.72467845
AATAGCG1605.820766E-1126.48559262
Produced by FastQC (version 0.11.5)