FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823152
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.045.0070761
GTATCAA24200.040.9831541
TATCAAC29600.034.177542
ATCAACG29600.033.945043
TCAACGC29650.033.8877944
CAACGCA30150.033.3258135
AACGCAG31350.032.1599436
CTCGTAT1001.4071702E-831.14307239
ACGCAGA33000.030.3434017
CGCAGAG33650.029.757278
AGAGTAC33750.029.4651911
CAGAGTA34100.029.26367210
GCAGAGT34900.028.7900649
GTACTAA1057.8021003E-726.237891
GAGTACG28750.025.972112
AGTACGG29050.025.58543613
TCGTATG1251.2181408E-724.93425840
GTACGGG31700.023.22948814
TGGTATC3900.022.9400272
TACGGGC7100.022.2938315
Produced by FastQC (version 0.11.5)