FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576989
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCGTGCTTGTATATGAA69510.26973339816351566No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGC66620.2585187596842672No Hit
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA62170.24125054472487079No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG59670.2315493003656593No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC57890.22464201438190073No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55780.21645416414272628No Hit
TCTCAAGACTCCGGGCTGAGGACGAGGCTGATTATTCCTGCAGTTCATAT51220.19875909443152454No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT50560.19619796592069272No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46460.18028792517158593No Hit
GTGTATTACTGTGCGAAATCGGAAATTGCCCTCCGTACTGGGAGCTACTA45270.17567013285660127No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC44290.17186724506779036No Hit
GTCTTGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG44180.17144039031598504No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40890.15867355273926276No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC40840.15847952785207853No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39620.15374532060478333No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAATGC37760.14652759480153No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36400.14125011787011899No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36280.1407844581408768No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34700.13465327170585517No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33930.13166528844321804No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC33830.13127723866884958No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA33440.12976384454881257No Hit
GTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG32260.12518485721126477No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32190.12491322236920686No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31800.12339982824916987No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31630.12274014363274348No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31000.12029543005422219No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30960.1201402101444748No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCG30480.1182775712275062No Hit
CACCTGCACTGTCGCTGGTGACTCCATCAGCAGTAGTGGTTACTACTGGG30310.11761788661107983No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30000.11641493231053762No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT29410.1141254386417637No Hit
GTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGA28560.11082701555963181No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28300.10981808614627382No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCA27810.10791664225186835No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27670.1073733725677525No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26850.10419136441793117No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25930.10062130649374135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)