FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561057
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCG451.7703424E-646.32994547
GGTATCA8300.045.274021
GTATCAA18900.040.1292951
CTCGTAT655.309794E-737.272939
CGTAGCA400.002544716534.63846641
TCGTATG806.859591E-834.62605340
TCAACGC23000.033.2459374
ATCAACG23300.032.9657063
CAACGCA23500.032.831725
TATCAAC23700.032.409322
AACGCAG24150.032.23336
TGCGTAT553.0836088E-431.65405550
ACGCAGA25150.030.9516567
TGCGTAG450.00454892630.76493339
GTATAGA450.004637295430.6441881
CGCAGAG25850.029.980268
CTTATAC1151.713488E-929.978011
AGAGTAC25700.029.88719611
CAGAGTA26250.029.26098810
TACGGGA7350.028.58616815
Produced by FastQC (version 0.11.5)