FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561057
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8000.14258800799205784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.040.9413221
GTATCAA22600.037.4341351
GTGGTAT3950.033.9553871
GCGCTAT750.002827341233.8974270
TCAACGC25950.032.32794
TATCAAC26250.032.2203942
CAACGCA26200.032.019435
AACGCAG26600.031.6728326
ATCAACG26550.031.5973243
TGGTATC4050.031.4102752
ACGCAGA27100.030.967097
CGCAGAG27450.030.8283378
AGAGTAC27600.030.53615411
CAGAGTA28500.029.81325510
GCAGAGT29850.028.3496749
AGTACGG26450.027.7020113
GAGTACG26750.027.64852512
CTATACA500.00786248227.5052154
GTACGGG29100.024.94289214
CACGCGG700.00135575324.57136510
Produced by FastQC (version 0.11.5)