FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752605
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC53540.7113957520877486No Hit
GTATAATACTTATCCGCTCACTTTCGGCGGAGGGACCAATGTGGAGATCAAACGAACTGTGGCTGCACCAT42080.559124640415623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23380.310654327303167No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19400.25777134087602394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17720.23544887424346103No Hit
GGATTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16960.22535061552873023No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16090.2137907667368673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15730.20900738102988953No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15640.20781153460314505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14500.19266414653104882No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14030.18641917074693895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14000.18602055527135747No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13610.18083855408879823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13460.17884547671089085No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13050.17339773187794394No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGA13050.17339773187794394No Hit
GTATTAATCCTCTGCCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA11900.15811747198065387No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACATTGGTCCCTCCGCCGAAAGTG11780.15652301007832795No Hit
ATACTTATCCGCTCACTTTCGGCGGAGGGACCAATGTGGAGATCAAACGA11520.15306834262328844No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGGATTAATACCTGGTTGGCCTGGTATCAGCAGAAA11370.15107526524538104No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA11320.1504109061194119No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11310.15027803429421807No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11250.14948080334305514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11240.1493479315178613No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10980.1458932640628218No Hit
GTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA10780.14323582755894526No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACCTATTACTGCCAACAGTATAATACTTAT10640.1413756220062317No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGGATTAATAC10550.14017977557948724No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10410.13831957002677367No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9770.12981577321436877No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9740.1294171577387873No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9670.12848705496243049No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9600.12755695218607369No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9250.12290643830428977No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9110.1210462327515762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8850.1175915652965367No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACGCAGTCTCCTTCCACC8540.11347253871552808No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8460.11240956411397746No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8450.11227669228878363No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8440.11214382046358981No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8370.11121371768723301No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8300.11028361491087622No Hit
GATAAGTATTATACTGTTGGCAGTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT8200.10895489665893796No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8160.10842340935816265No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8040.10682894745583672No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTATTA8030.1066960756306429No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7570.10058397167172688No Hit
ACCTTGATGGGACCCCACTTTGTAAATTAGACGCCTGATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG7570.10058397167172688No Hit
ACCTATTACTGCCAACAGTATAATACTTATCCGCTCACTTTCGGCGGAGGGACCAATGTGGAGATCAAACGAA7540.1001853561961454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)