FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752605
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC50740.6741916410334771No Hit
GTATAATACTTATCCGCTCACTTTCGGCGGAGGGACCAATGTGGAGATCAAACGAACTGTGGCTGCACCAT37140.4934859587698726No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24270.32247991974541756No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18110.24063087542602027No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17960.2386377980481129No Hit
GGATTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT14590.19385999295779327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14370.1909368128035291No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14240.18920947907600932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13990.18588768344616366No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13740.182565887816318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13210.17552368108104516No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12960.17220188545119952No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12450.16542542236631433No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11930.15851608745623536No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGA11430.151872496196544No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11330.15054377794460572No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11240.1493479315178613No Hit
GTATTAATCCTCTGCCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA11190.14868357239189217No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11150.14815208509111685No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACATTGGTCCCTCCGCCGAAAGTG10810.14363444303452674No Hit
ATACTTATCCGCTCACTTTCGGCGGAGGGACCAATGTGGAGATCAAACGA10760.14297008390855762No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGGATTAATAC10720.1424385966077823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10560.14031264740468108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10530.1399140319290996No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA10340.1373894672504169No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGGATTAATACCTGGTTGGCCTGGTATCAGCAGAAA10180.13526351804731565No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10170.13513064622212184No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9960.13234033789305147No Hit
GTAATAGGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA9680.12861992678762432No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9620.12782269583646136No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9600.12755695218607369No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9230.12264069465390212No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9130.12131197640196385No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9040.12011612997521941No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9030.11998325815002558No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACCTATTACTGCCAACAGTATAATACTTAT9030.11998325815002558No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9020.11985038632483175No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8900.11825592442250582No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACGCAGTCTCCTTCCACC8800.11692720617056755No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8720.11586423156901694No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8500.11294105141475277No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8490.11280817958955894No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8260.10975212761010092No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8150.10829053753296883No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTATTA7540.1001853561961454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT252.5930838E-455.0854644
CGGTTAC200.00683631952.7214365
GTATCAA13100.043.3697971
TATTAGA503.896257E-641.31412
TATCAAC14850.037.790112
GTGGTAT1850.037.2248541
ATCAACG15850.035.4058763
CAACGCA15950.035.18395
TCAACGC16000.035.0739484
AACGCAG16300.034.437576
AGAGTAC17950.031.29695711
CGCAGAG18050.031.1235668
ACGCAGA18150.030.9479687
GCAGAGT19000.029.9301749
TATACTG14200.029.5793425
GTATTAT14150.029.4444641
ATTATAC14200.029.3368873
TATTATA14250.029.233952
TTATACT14300.029.1317354
GCAGTAA13850.029.1148815
Produced by FastQC (version 0.11.5)