FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562064
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70780.45311843816898667No Hit
GTATGGTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGAACTGTGGCTGCACCAT62490.40004762929047716No Hit
CTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC56740.3632373577523072No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51190.32770744348503006No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC49980.3199612819961282No Hit
GTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGA48250.30888619160290487No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44940.2876962787696279No Hit
GATTACTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACT44570.2853276178184761No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44090.2822547603683332No Hit
CCTTCCACCCTGTCTGCATCTATGGGAGACAGAGTCACCATCACTTGCCG42770.27380440238044024No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGGTAGTAAT41440.2652900265290027No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG40920.26196109762468117No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40770.2610008296715115No Hit
GTCTGCATCTATGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA39920.25555931127021686No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39330.2517822573210829No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39160.250693953640824No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37880.24249966710710957No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT36220.23187270175869876No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34570.2213097542738326No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33980.2175327003246986No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCGAACGAACTGTGGCTGCACCA32320.2069057349762878No Hit
GTGCAGCCACAGTTCGTTCGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG31820.20370484179905562No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC31140.19935162707801984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30440.19487037662989481No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30120.19282180499646623No Hit
GGCCAAAAGTGTACGGATTACTACCATACTGTTGGCAGTAATAAGTTGCA29670.18994100113695728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29030.18584385787010008No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC28100.17989019656044825No Hit
GTCAGAGTGTTCGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGG28080.17976216083335894No Hit
GATCTATAAGGCGTCGACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA27760.17771358919993036No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27140.17374448166016246No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26880.17208001720800173No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26610.17035153489229635No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATGTCCAGCTTGGTCC26350.16868707044013562No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26190.16766278462342132No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26100.1670866238515195No Hit
GTCTCCTTCCACCCTGTCTGCATCTATGGGAGACAGAGTCACCATCACTT25130.1608768910876891No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24620.1576119800469123No Hit
ATGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTCGTAGTTGGTTGGCCTGGTATCAGCAGAAA24110.15434706900613548No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23980.15351483678005512No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23250.14884153274129613No Hit
CAGTATGGTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGAACTGTGGCTGCACCAT23230.14871349701420683No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22660.14506447879216217No Hit
ACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC22640.14493644306507286No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT22610.14474438947443896No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCGACTTTAGAAAGTGGGGTCCCAT22290.14269581784101038No Hit
GTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGATTACTACCATACTGTT21540.13789447807516209No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21480.13751037089389423No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21480.13751037089389423No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21180.13558983498755492No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21040.13469358489792993No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20750.13283706685513527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20590.13181278103842098No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC19750.1264352805006709No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACGA19630.1256670661381352No Hit
GCCACAGTTCGTTCGATGTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG19500.12483483391205483No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACGAACACTCTGA19380.12406661954951911No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19140.12253019082444766No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTATGGGAGA19070.12208206577963515No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19040.12189001218900122No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTCGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG18670.1195213512378494No Hit
TTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC18610.11913724405658153No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18390.1177288510585994No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18280.11702465455960831No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCGACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT17990.11516813651681365No Hit
TCTATAAGGCGTCGACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT17620.11279947556566185No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGC17360.1111350111135011No Hit
GTTCGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA17310.11081492179577788No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17030.10902242161652786No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17010.10889438588943859No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16990.1087663501623493No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC16850.1078701000727243No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16620.1063976892111975No Hit
TTCTAAAGTCGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT16610.10633367134765286No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16610.10633367134765286No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC16600.10626965348410822No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGT16540.10588554630284035No Hit
AAGCTGGACATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16500.10562947484866177No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG16320.1044771533048582No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16290.10428509971422426No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16160.1034528674881439No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15760.10089215294635816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35000.044.7108541
GTGGTAT3500.043.236871
TATCAAC40950.038.2726442
ATCAACG42050.037.434933
TCAACGC42150.037.3461154
CAACGCA42800.036.7789425
AACGCAG44350.035.648546
ACGCAGA46650.033.888787
CGCAGAG46800.033.7812428
AGAGTAC46950.033.6011911
TCGACGT1350.033.128729
CAGAGTA48150.032.90653610
CTCGACG1400.031.93836828
GCAGAGT50100.031.6933299
AGTACGG39500.030.19325313
GAGTACG40200.029.83849512
CGGGATT1401.4551915E-1129.46080817
ACTCGAC1551.8189894E-1228.84016827
ACGGGAT2550.026.95760216
TTCGACG657.9900766E-426.90602356
Produced by FastQC (version 0.11.5)