FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224575
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA28480.23257048363718025No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23140.18896351795520896No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG22740.18569707857828227No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATGCCACGAGTGTGCTCGTGCTTGTATATGAA22600.18455382479635793No Hit
TCTCAAGACTCCGGGCTGAGGACGAGGCTGATTATTCCTGCAGTTCATAT20290.16569013739460628No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATGCCACGAGTGTGCTC20120.16430190065941244No Hit
GTGTATTACTGTGCGAGATCGGAAATTGCCCTCCGTACTGGGATCTACTA19450.15883061470306023No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT19160.1564624461547884No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18810.15360431169997754No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18580.15172610905824468No Hit
GTCTTGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG17130.13988526631688547No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16730.13661882693995878No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16450.13433231937611007No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC15530.1268195088091787No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15500.1265745258559092No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15020.12265479860359718No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAATGC14990.12240981565032766No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14870.12142988383724966No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14860.1213482228528265No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14450.11800012249147662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13800.11269215850397075No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13740.11220219259743176No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13240.1081191433762734No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGCGT12880.10517934793703937No Hit
GTTCATATACAAGCACGAGCACACTCGTGGCATTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG12870.10509768695261622No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12740.10403609415511504No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA12680.10354612824857604No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12640.10321948431088337No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12530.10232121348222852No Hit
CACCTGCACTGTCGCTGGTGACTCCATCAGCAGTAGTGGTTACTACTGGG12470.10183124757568952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)