FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444851
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16020.36012057969971967No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9470.21288026777505276No Hit
GTATTACTGTCAGCAGTATGGAATCTCGCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAA9000.20231493241557286No Hit
GTATGGAATCTCGCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAACTGTGGCTGCACCAT7220.16230153467115957No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7040.1582552360228481No Hit
CAGTAATACACTGCAAAATCATCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6670.14993784435687454No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT6340.14251963016830355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6220.1398220977360959No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5910.13285347228622618No Hit
GTGTATTACTGTCAGCAGTATGGAATCTCGCCCTCGTACACTTTTGGCCA5890.1324038835475249No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5790.13015593985401852No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA5710.12835758489921345No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAACTGTGGCTGCACCA5600.12588484683635645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5520.12408649188155134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5410.12161375381869435No Hit
GGTTTATGTTGGTACCAGGCTAAATAGCTGCTGCTAATAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC5310.11936581012518799No Hit
ACATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCGGACAGGTT5110.11486992273817526No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAG5020.11284677341401952No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4990.11217239030596761No Hit
ATTCCATACTGCTGACAGTAATACACTGCAAAATCATCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC4860.10925006350440934No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCAGTGGCCCTGCT4610.10363020427064343No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4600.10340540990129281No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4590.10318061553194215No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGATGATT4590.10318061553194215No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4530.10183184931583833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)