FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444851
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30410.6835996771952856No Hit
GTATTACTGTCAGCAGTATGGAATCTCGCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAA8860.19916781124466396No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8540.1919743914254436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7030.15803044165349747No Hit
GTATGGAATCTCGCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAACTGTGGCTGCACCAT6910.15533290922128984No Hit
CAGTAATACACTGCAAAATCATCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6280.14117086395219972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6160.13847333151999208No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT6060.13622538782648572No Hit
GTGTATTACTGTCAGCAGTATGGAATCTCGCCCTCGTACACTTTTGGCCA5900.13262867791687555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5870.13195429480882365No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA5770.12970635111531728No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5640.126784024313759No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAACTGTGGCTGCACCA5590.12566005246700582No Hit
GGTTTATGTTGGTACCAGGCTAAATAGCTGCTGCTAATAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC5310.11936581012518799No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5160.11599389458492844No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5060.11374595089142206No Hit
ACATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCGGACAGGTT5000.11239718467531826No Hit
ATTCCATACTGCTGACAGTAATACACTGCAAAATCATCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC4800.10790129728830553No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGATGATT4780.10745170854960424No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4740.1065525310722017No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAG4710.1058781479641498No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4670.10497897048674726No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4630.1040797930093447No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCAGTGGCCCTGCT4550.10228143805453961No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCAGCAGCTATTTAGCCTGGTACC4470.10048308309973451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG150.002408044968.6104830
TCAGTAT200.00752257551.4578630
GGTATCA4650.045.6272161
GTATCAA10500.037.47951
ATTACGG400.002654418634.3400631
TAACCTA400.002700246634.220415
TATCAAC11600.033.9254072
TCAACGC12450.031.6092154
GTGTTAT553.4128464E-431.1094651
ATCAACG12650.031.1094653
CAACGCA12700.030.9869885
AACGCAG12850.030.6321566
AGAGTAC13700.028.7542511
AATGGTA1604.241154E-628.55322870
TATACTA605.681059E-428.5170085
CGGATAG500.00693529528.22004360
TATACGG854.709038E-628.1815155
CGCAGAG14050.028.0379528
TCGAGGG500.007760247727.57780835
GTAAGTA500.00804297727.3763283
Produced by FastQC (version 0.11.5)