FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051027
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11610.11046338486071243No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11260.10713330865905442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.045.1262441
GTATCAA22850.037.010771
TATCAAC26550.031.4434662
ATCAACG26700.031.3960193
CAACGCA27150.030.7485835
TCAACGC27200.030.6920594
AACGCAG27900.029.9220096
GTGGTAT4550.028.0697521
ACGCAGA30000.027.8274677
ACGGGTA756.162843E-527.60011516
CGCAGAG30800.027.1046738
AGAGTAC32300.025.74159211
GCAGAGT33450.025.0605019
CAGAGTA33950.024.7953910
AGTACGG25250.023.91099213
GAGTACG28550.021.2680412
GTACGGG28750.020.88008714
ACGGGAT2850.020.57903116
TTCGGGG4651.8189894E-1220.49154370
TACGGGA9600.020.12508215
Produced by FastQC (version 0.11.5)