FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542479
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT6760.1246131186645013No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6240.11502749415184736No Hit
GTATTACTGTCAGCAGTATGGAATCTCGCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAAGAA6040.1113407154931343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAG200.007409108351.65759333
GGTATCA8050.048.644011
TCGTATG1500.048.49164240
CTCGTAT1600.045.4271339
CGTATGC1650.044.13254541
TATGCCG1800.042.45236643
ACGAGAC2700.040.71802522
GTATCAA19900.040.390871
CTTATAC2900.039.087361
GCCGTCT1700.038.92167746
ATCTCCG3050.038.28771210
CGAGCCC3150.035.98518815
CCGAGCC3150.035.98187314
TATCAAC22600.035.406892
TAGTTCG400.002383476135.10354257
CGTCTTA601.2434442E-534.88334748
TATACAC3550.034.8268743
CCCACGA3200.034.35266519
TCCGAGC3300.034.34633313
TCTCCGA3450.033.84855711
Produced by FastQC (version 0.11.5)