FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319476
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40900.043.4532281
GGTATCA14850.038.8899731
TATCAAC46900.037.151362
ATCAACG47700.036.3121343
TCAACGC48800.035.4936184
TCGATTA400.002335701135.25252564
CAACGCA49250.035.0995335
AACGCAG50950.033.934836
ACGCAGA53250.032.4143757
AGAGTAC54600.031.87086311
CGCAGAG54650.031.7146428
GTGGTAT6400.031.6903341
CAGAGTA56250.030.87484610
GCAGAGT58450.029.7127469
TGGTATC6650.028.4237022
AGTACGG44550.028.09891913
GAGTACG45700.027.3165812
GTACGGG49950.024.99140214
GAGTACT20000.024.76101512
AGTACTT20750.023.700313
Produced by FastQC (version 0.11.5)