FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416085
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTACTCCTATCCCACAC44320.3129755629075938No Hit
CCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG38670.27307682801526745No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35740.25238597965517606No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTA35660.2518210418159927No Hit
GGATTACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC33850.23903932320446866No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTC29280.20676724914111796No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28810.20344823933591558No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26700.18854800382745387No Hit
GGGTGGCCGTGTTCCCAGAATTGGAGCCAGACAATCGCTCAGGGATCCCTGAGGGCCGTTTACTATCCTCATAGA25990.18353418050470136No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGT25160.17767295042317374No Hit
CTCCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC25010.17661369197470492No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24810.17520134737674645No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGTAACAACATTGGAAGT24520.1731534477097067No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24380.17216480649113577No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24360.17202357203133992No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA24210.17096431358287106No Hit
GTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG23850.16842209330654587No Hit
CCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCT22610.15966555679920344No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGC22380.1580413605115512No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22320.1576176571321637No Hit
GGATAGGAGTAGTGATCTTATAATTTTCGGCGGAGGGACCAAGATGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22010.15542852300532808No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21900.15465173347645092No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA21600.15253321657951324No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20740.14646013480829187No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20730.14638951757839394No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCAC20200.14264680439380403No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20050.14158754594533518No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT19760.13953964627829546No Hit
GTATATTACTGTGCGAAACAGACGGACGTGGGAGATTACAATTTTTGGAGTGGTTATCCGGGGCACTTGGATTA19620.13855100505972454No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19590.13833915337003075No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18780.132619157748299No Hit
GGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC18670.13184236821942186No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18460.13035940639156549No Hit
GTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCG18440.13021817193176963No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18240.12880582733381118No Hit
GCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCA18110.1278878033451382No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18070.1276053344255465No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17820.1258399036780984No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT17700.12499249691932333No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG17650.12463941076983373No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC17350.12252089387289604No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17260.12188533880381475No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16790.11856632899861236No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16780.11849571176871444No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16420.11595349149238923No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16260.11482361581402246No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA16230.11461176412432869No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16190.11432929520473699No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16140.11397620905524738No Hit
CTACGCAGACTCCGTGAAGGGCCAATTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTGCAAATGAA16000.11298756783667648No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAATTGGAGCCAGA15860.11199892661810555No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15830.11178707492841178No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGG15820.11171645769851386No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15620.11030411310055539No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTAGCAGTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15620.11030411310055539No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT15500.10945670634178034No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15170.10712633775514888No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14780.10437226578912989No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14710.10387794517984442No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14640.10338362457055898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.045.3587881
GTGGTAT3550.041.7556341
TGGTATC3650.040.595872
GTATCAA27200.040.429351
TATCAAC31150.035.2889672
ATCAACG32100.034.3519363
TCAACGC32200.034.2452554
CAACGCA32300.034.139235
AACGCAG33150.033.5780876
AGAGTAC34750.032.0478911
CAGAGTA35050.031.77358810
CGCAGAG35250.031.4954998
GCAGAGT36250.030.7217719
AATATAC10450.029.0183493
TATACGG10550.028.0900365
ATACGGC10450.028.0310676
TCTGGAG16050.027.80595470
TACGGGT2250.027.58106615
AGTACGG33300.027.4380613
TACGGCC10600.027.3189377
Produced by FastQC (version 0.11.5)