FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645449
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT19780.12021034988018468No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC18580.11291750762253949No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT17390.10568543905037471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45600.042.637091
GGTATCA17750.039.230721
ATCAACG52000.037.0332073
TCAACGC52900.036.3369264
CAACGCA53700.035.859745
AACGCAG55450.034.7280126
TATCAAC56050.034.2343752
ACGCAGA59100.032.69887
CGCAGAG59450.032.623178
AGAGTAC60650.031.97867211
CAGAGTA61850.031.35822710
GCAGAGT65850.029.5580319
GAGTACG50850.027.97962612
AGTACGG50700.027.92651213
TACGGGG27150.025.63015215
GTGGTAT7100.024.7619651
GTACGGG56800.024.74540914
GAGTACT23450.022.32973512
GACCGGA2754.1103704E-822.2446970
GTAGTTT9750.021.95950370
Produced by FastQC (version 0.11.5)