FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645449
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20010.12160814464623335No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT19380.11777940246096963No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC18480.11230977076773574No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT17320.10526002325201207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46400.041.000451
GGTATCA19450.037.6739771
GTGGTAT7350.036.976111
ATCAACG54850.034.4248123
TCAACGC55300.034.082494
CAACGCA55800.033.7154545
TATCAAC56300.033.4771162
AACGCAG57450.032.8109786
ACGCAGA60150.031.2923957
CGCAGAG60850.030.9927338
ACGCGTT450.00467910630.5914911
AGAGTAC63400.029.74708411
CAGAGTA64400.029.39205210
GCAGAGT66150.028.7176659
TGGTATC9250.027.5147692
AGTACGG50250.027.25838713
GAGTACG51050.026.8312212
GTAGTTT10100.026.08389770
TCGCTAG700.001221094725.01989658
TAACGGC7850.023.76245536
Produced by FastQC (version 0.11.5)