FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073393
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28270.2633704523878952No Hit
GTAATAGACAGCCGTGTCCTCAGTTCTCGGGCTGATCACATGCAGATACA27830.2592713013779669No Hit
GTCTATTACTGTGCGAAAGGTGTTCATAGTGGCTACAGTGTGTTCCAGTGGGGACCAAAATCCCCGAATCCCCTT26720.24893026133019314No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA23090.2151122654982844No Hit
GTATTACTGTCAGCAGTATGAGAACTCAGTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGG22960.2139011526998965No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19140.17831306893188237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17800.16582929085619152No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17490.16294125264465112No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17060.1589352641576757No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT16380.1526002125968774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16050.14952584933943114No Hit
GATCAGCACTGACCACAGAGGACTCACCATGGAGCTTGGGCTGAGCTGGG15660.1458925109442674No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15590.14524037328359699No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15300.1425386601179624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15120.1408617347048099No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14230.13257027016200032No Hit
TCTATGATGTGTCCAACAGGGCCAGTGGCATCCCAGACAGGTTCAGAGGCAGTGGGTCTGGGACAGACTTCACT14180.1321044575472357No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14130.13163864493247115No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATT13960.13005488204227156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13560.1263283811241549No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12990.12101811731583866No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12930.12045914217812116No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12750.11878221676496865No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12610.11747794144362782No Hit
GTGTATTACTGTCAGCAGTATGAGAACTCAGTCACTTTCGGCGGGGGGAC12310.11468306575504032No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12190.11356511547960532No Hit
GTATGAGAACTCAGTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT12000.11179502754349992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11950.11132921492873532No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11840.11030442717625324No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11760.10955912699262992No Hit
GTCCCAGACCCACTGCCTCTGAACCTGTCTGGGATGCCACTGGCCCTGTT11740.10937280194672408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11620.10825485167128908No Hit
CTCTTGTGCAGCCTCTGGACTAAGCCTCAGAAACTACGCCATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGG11490.10704373887290117No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11450.1066710887810895No Hit
GACTATTACCAGCACCTACTTGGCCTGGTACCAGCAGAAACCTGGCCAGG11340.10564630102860742No Hit
GTTCTCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGT11260.10490100084498408No Hit
GTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGGCGTAGTTTC11050.10294458786297284No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC11020.10266510029411408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.048.574631
GTATCAA26300.041.7167631
GTGGTAT2950.038.4740221
TATCAAC30050.036.3896062
TGGTATC3200.034.3870352
ATCAACG32500.033.6463933
CAACGCA33000.033.13665
TCAACGC32850.033.078554
AACGCAG33100.033.0411076
AGAGTAC35400.030.70864711
CGCAGAG36250.030.0820838
ACGCAGA36700.029.805577
CAGAGTA37300.028.9599410
GTACGGA605.5209914E-428.6612051
CTATACC1103.781315E-828.1348484
GCGTGTT500.007699972527.62544836
AGTACGG31950.026.48750913
GTCTAAT658.8144955E-426.4564951
GAGTACG32250.026.13444112
TTAATCG1151.6791109E-624.02999937
Produced by FastQC (version 0.11.5)