FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194379
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35650.040.8366551
GGTATCA15000.039.9233861
TATTACG451.0136921E-438.25521526
GTGGTAT4400.036.76331
TATCAAC41800.034.4716072
TCAACGC42150.034.0221864
ATCAACG42600.033.824253
CAACGCA43200.033.3544655
AACGCAG46250.031.3035876
ACGCAGA48150.030.0683487
AGAGTAC49250.029.33062711
CGCAGAG49900.028.8772228
CAGAGTA49950.028.84952410
GCAGAGT52550.027.487599
TGGTATC5950.027.1646842
AGTACGG36900.025.5389213
GAGTACG37000.025.37693812
TACGGGG18550.023.73155415
GTACGGG40050.023.44436314
GAGTACT22400.022.57083312
Produced by FastQC (version 0.11.5)