FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278880
Sequences flagged as poor quality0
Sequence length21-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGCTGTCATAGGACTGG58550.45782246966095336No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGCTGTCATAGGACTGGCAGTAATAAT51350.40152320780683104No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAACCTGGAGCCCAG45370.3547635431002127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41580.32512823720755657No Hit
CTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGC37780.2954147378956587No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37080.28994119854873013No Hit
GGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGG35720.27930689353184035No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35420.27696109095458527No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35020.2738333541849118No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31820.2488114600275241No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31330.24497998248467406No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31040.24271237332666082No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30080.23520580507944452No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29620.23160890779432003No Hit
GTCCTATGACAGCAGCCTAAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC29390.2298104591517578No Hit
TCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCCTCAGGGGGCCCTGACCGATTCTCTGG29070.227308269736019No Hit
CACTTAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCA28560.22332040535468534No Hit
GTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC28020.2190979607156262No Hit
CTCCTGTACAGCTTCTGGATTCATTTTTGGTGACTATGTCATAAATTGGATCCGCCAGGCTCCAGGGAAGGGG27920.2183160265232078No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27840.21769047916927312No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27110.21198235956461903No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26610.2080726886025272No Hit
GGTCATCACTGGGCTCCAGGTTGAGGATGAGGCTGATTATTACTGCCAGT26430.20666520705617414No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26360.2061178531214813No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24750.1935287126235456No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24530.1918084574002252No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24400.19079194295008134No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24370.1905573626923558No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23780.18594395095708746No Hit
GCCTAAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT23460.18344176154134867No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23420.18312898786438134No Hit
GGACAGAGGGTCATCATCTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAG23150.18101776554485174No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22720.17765544851745277No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22090.17272926310521708No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22010.17210371575128236No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21960.1717127486550732No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21910.171321781558864No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGC21470.16788127111222317No Hit
GTTCCTGGAAGGTGCTGATACCAATGTACACCATAGCCTGCCCCGATGTT21280.1663955961466283No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT20720.16201676466908546No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC20600.1610784436381834No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20390.15943638183410486No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC20330.15896722131865382No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19150.1497403978481171No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19020.1487238833979732No Hit
ATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCCT18920.14794194920555487No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18880.1476291755285875No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTAAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18730.14645627423995997No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18700.14622169398223447No Hit
TCACTGGGCTCCAGGTTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT18540.14497059927436506No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18470.1444232453396722No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACA18260.14278118353559366No Hit
GTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTC18180.14215563618165894No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18160.1419992493431753No Hit
CCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCAGGTCCAACATC17960.14043538095833855No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17920.14012260728137121No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17870.13973164018516201No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17760.1388715125735018No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17730.1386369323157763No Hit
ACCTGGAGCCCAGTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCC17450.13644751657700488No Hit
GTAATAATCAGCCTCATCCTCAACCTGGAGCCCAGTGATGACCAGGGAGG17440.13636932315776304No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17020.1330851995496059No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16960.1326160390341549No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16960.1326160390341549No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16860.13183410484173652No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16770.13113036406856No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16610.1298792693606906No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16590.12972288252220693No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16510.12909733516827224No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGG16440.12854998123357939No Hit
GTCTGGCACCTCAGCCTCCCTGGTCATCACTGGGCTCCAGGTTGAGGATG16240.12698611284874267No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16070.12565682472163142No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15950.12471850369072938No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15880.12417114975603652No Hit
GTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCT15730.12299824846740898No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG15700.12276366820968349No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15520.12135618666333041No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC15410.12049605905167021No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15160.1185412235706243No Hit
GGTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCT15120.11822844989365697No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15040.11760290253972226No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14940.11682096834730389No Hit
GGGCCCCCTGAGGGCCGATTGTTGTTACTAGAGACGAGGAGTTTGGGGGC14930.11674277492806207No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14910.11658638808957839No Hit
GCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCCTCAGGGGGCCCTGACCGATTCTCTGGCTCCAAGT14840.11603903415488552No Hit
CAGGGGGCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14810.11580445389716001No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14720.11510071312398348No Hit
GCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAA14380.11244213686976104No Hit
TTCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCC14360.11228575003127737No Hit
GTGTTAGGCAGATTCTTTATCTCAAGAGATGATTCTAAAAGCATCGCCTT14090.11017452771174777No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14080.11009633429250594No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14030.10970536719629677No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCATTTTTGGTGACTATGTC13830.10814149881146003No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13780.10775053171525086No Hit
GTGTCCGGGGCCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCAG13780.10775053171525086No Hit
GCCCAGTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT13780.10775053171525086No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG13590.10626485674965595No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13540.10587388965344675No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13500.10556111597647942No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13480.10540472913799576No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG13460.10524834229951208No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13290.10391905417240085No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13260.10368447391467535No Hit
GCCCTCAGGGGGCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG13240.10352808707619167No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13170.1029807331414988No Hit
GCCTAACACAGACGCGGCGTGTTCTGTCGTCCCACCATAAACCTGGCTTCTCATGAAACCTACCCACTCCAGCCC13120.10258976604528963No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13050.10204241211059677No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC12920.1010258976604529No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA12900.10086951082196924No Hit
GGGCTGGAGTGGGTAGGTTTCATGAGAAGCCAGGTTTATGGTGGGACGAC12840.1004003503065182No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC12830.10032215688727637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT200.007331338251.7997127
GTGGTAT2250.047.597611
TATCAAC22950.036.71212
ACATAGG400.002520941434.7082251
TGTATAA1003.783498E-1034.52773714
GTGTATA1003.783498E-1034.52773713
ATCAACG24500.034.248563
GTATCAA25650.033.940571
CAACGCA24650.033.9000665
TCAACGC24600.033.82864
AACGCAG24900.033.5597046
GAAGCCG7800.032.57142670
GCTTGTA851.1943484E-732.5144961
CGCAATC652.1260152E-532.25896562
AGAGTAC26050.031.9431311
CAGAGTA25950.031.80011410
CGCAGAG26200.031.6284668
CAGTGGT3500.031.5706884
TATAAGA1101.0495569E-931.38885516
GCAGAGT26750.030.9781599
Produced by FastQC (version 0.11.5)