FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278880
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGCTGTCATAGGACTGG48360.37814337545352184No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGCTGTCATAGGACTGGCAGTAATAAT44630.34897723007631676No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41690.32598836481921684No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCCGGGGCCCCAGGACAGAGGGTCATCATCTCCTG38380.3001063430501689No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAACCTGGAGCCCAG36280.2836857250093832No Hit
CTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGC35570.2781339922432128No Hit
GGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGG34200.2674214938070812No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34120.2667959464531465No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33450.26155698736394345No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33140.25913299136744655No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31640.24740397848117104No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCCGGGGCCCCAGGACAGAGGGTCAT30780.24067934442637307No Hit
GTCCTATGACAGCAGCCTAAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC29680.23207806830977104No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29000.22676091580132615No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28730.2246496934817966No Hit
CTCCTGTACAGCTTCTGGATTCATTTTTGGTGACTATGTCATAAATTGGATCCGCCAGGCTCCAGGGAAGGGG28590.22355498561241086No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28140.2200362817465282No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27490.21495370949580883No Hit
TCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCCTCAGGGGGCCCTGACCGATTCTCTGG27200.21268610033779559No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27050.21151319904916802No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26920.21049668459902415No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26790.20948017014888026No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25370.19837670461653947No Hit
GGTCATCACTGGGCTCCAGGTTGAGGATGAGGCTGATTATTACTGCCAGT25270.1975947704241211No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24590.1922776179156762No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCCGGGGCCCCAGGACAGAGGGTCAT23770.18586575753784562No Hit
GGACAGAGGGTCATCATCTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAG23380.182816214187414No Hit
GTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC23380.182816214187414No Hit
GCCTAAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT23120.18078318528712622No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22930.17929751032153135No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22710.17757725509821096No Hit
CACTTAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCA22440.17546603277868134No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21680.16952333291630176No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21440.16764669085449768No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21150.16537908169648444No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20760.1623295383460528No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20760.1623295383460528No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC20240.1582634805454773No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20080.1570123858376079No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT19930.15583948454898036No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19750.1544320030026273No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19730.15427561616414362No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC19510.15255536094082323No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19310.15099149255598648No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCC19190.15005317152508446No Hit
GTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTC18590.14536156637057426No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18540.14497059927436506No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCCGGGGCCCCAGGACAGAGGG18510.14473601901663957No Hit
ATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCCT18480.14450143875891405No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTAAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18410.1439540848242212No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18210.14239021643938446No Hit
GTTCCTGGAAGGTGCTGATACCAATGTACACCATAGCCTGCCCCGATGTT18170.14207744276241713No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17950.1403571875390967No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17950.1403571875390967No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17600.13762041786563242No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17520.13699487051169773No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGGCTGC17120.13386713374202427No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17110.13378894032278243No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16790.13128675090704364No Hit
TCACTGGGCTCCAGGTTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT16700.13058301013386714No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16660.13027023645689978No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16600.12980107594144877No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16400.12823720755661203No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCCGGGGCCCCAGGAC16400.12823720755661203No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16370.12800262729888653No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16190.12659514575253347No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16180.12651695233329163No Hit
GTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCT16110.12596959839859875No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16080.12573501814087326No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16040.12542224446390593No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15980.12495308394845489No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15940.12464031027148756No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15790.12346740898286002No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15600.12198173401726511No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15540.12151257350181408No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15520.12135618666333041No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG15370.12018328537470287No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15330.11987051169773551No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15310.11971412485925184No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15300.11963593144001No Hit
GTCTGGCACCTCAGCCTCCCTGGTCATCACTGGGCTCCAGGTTGAGGATG15280.11947954460152634No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACA15170.11861941698986614No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15140.11838483673214063No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15040.11760290253972226No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTTAGG14840.11603903415488552No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG14830.1159608407356437No Hit
GTGTTAGGCAGATTCTTTATCTCAAGAGATGATTCTAAAAGCATCGCCTT14700.11494432628549982No Hit
GGGCTGGAGTGGGTAGGTTTCATGAGAAGCCAGGTTTATGGTGGGACGAC14600.11416239209308145No Hit
GGTCCAACATCGGGGCAGGCTATGGTGTACATTGGTATCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCGTCT14600.11416239209308145No Hit
CCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCAGGTCCAACATC14580.11400600525459778No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14450.1129894908044539No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC14390.11252033028900288No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14330.11205116977355185No Hit
TTCCAGGAACAGCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCC14110.11033091455023145No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14090.11017452771174777No Hit
CAGGGGGCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13980.10931440010008757No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13930.1089234330038784No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCCGGGGCCCCAGGACAGAGGGTCATCATCTC13780.10775053171525086No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13670.10689040410359066No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13570.10610846991117229No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCATTTTTGGTGACTATGTC13450.10517014888027025No Hit
GTGTCCGGGGCCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCAG13410.10485737520330289No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13380.10462279494557739No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13360.1044664081070937No Hit
GCCCCCAAACTCCTCGTCTCTAGTAACAACAATCGGCCCTCAGGGGGCCCTGACCGATTCTCTGGCTCCAAGT13350.10438821468785188No Hit
GTAATAATCAGCCTCATCCTCAACCTGGAGCCCAGTGATGACCAGGGAGG13330.10423182784936819No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13320.10415363443012635No Hit
ACCTGGAGCCCAGTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCC12880.10071312398348554No Hit
CCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGCTATGGTGTACATTGGT12870.1006349305642437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1950.051.2813451
GTAGTTT400.00782482950.95030670
GGGCAGT3200.036.6374241
TATCAAC24300.036.60082
ATCAACG25400.035.1514473
CAACGCA25700.034.741125
TCAACGC25700.034.741124
AACGCAG26300.034.0902826
GTATCAA27200.033.4680981
CGCAGAG27200.033.104618
AGAGTAC27200.032.9777711
CAGAGTA27550.032.5588210
AATCGTT652.2952288E-531.89966439
GCAGAGT28650.031.5495789
TTAACGG553.232739E-431.40402635
TTTAACG553.2357112E-431.39910734
AGTGGTG4050.030.642535
GGCAGTG3950.030.5455592
CAGTGGT4250.030.0116564
CAAACGA703.710774E-529.7329949
Produced by FastQC (version 0.11.5)