FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680183
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64040.3811489581789603No Hit
CTTTTATACAGGCCCAATAATAAGAACTACTTGGCTTGGTACCAGCAGAA55600.33091633470877874No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52620.3131801714456104No Hit
CTCCTAAGCTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT51690.3076450600916686No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46580.2772317063081819No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44530.26503065439895535No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43860.2610429935310618No Hit
GCTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCCAAGTAGTTCTTATTATTGGGCCTGTATAAAAGACT43830.2608644415519024No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG42100.2505679440870429No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41560.24735400846217348No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41320.24592559262889818No Hit
GTAGTAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATACA40600.24164034512907223No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTGGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA40330.24003337731663751No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39100.23271274617110158No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38890.2314628823169857No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGGAGTAGTATAAAATTGCTGACAGGA35210.2095605062067644No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC35160.20926291957483203No Hit
GTATAAAATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34120.20307311763063907No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32680.19450262263098722No Hit
GTCTACTACTGTGCGAGAGATTCTGGTGGGAGCTACGTTGTCGTTTGGGA32590.193966966693509No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTCTTTTATA31800.18926509790897778No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT31750.18896751127704542No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC31400.18688440485351893No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31230.18587261030494892No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30800.18331336527033068No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30540.18176591478428242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30510.18158736280512303No Hit
ATTTTATACTACTCCCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT30320.18045653360378006No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC30290.18027798162462064No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29910.17801632322193475No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29250.17408817968042767No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28170.1676603084306888No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT28070.16706513516682409No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27280.16236326638229287No Hit
GGAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26580.1581970535352399No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGCTTAGGAGGCTGTCGTGGTTTCTGCTGGTA26300.15653056839641874No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25370.15099545704247694No Hit
GTTTATTCCTGTCAGCAATTTTATACTACTCCCACTTTCGGCGGAGGGAC25250.1502812491258393No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25070.14920993725088277No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCC24860.1479600733967669No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24640.14665069221626453No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24580.1462935882579457No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24090.14337723926500864No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24070.1432582046122357No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23690.1409965462095498No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCAGCAATTTTATACTACTCCCACTTTCGG23440.13950861304988801No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC23200.13808019721661272No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCAGCAATTTTATACTACT22490.13385446704317328No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22480.1337949497167868No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21710.12921211558502854No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG21600.12855742499477735No Hit
GTAAATGAGTAGCTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCCA21530.12814080371007205No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21490.12790273440452618No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20980.12486735075881615No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT20910.12445072947411086No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20870.12421266016856497No Hit
ATCCACTGCAAGTCCAGCCAGAGTCTTTTATACAGGCCCAATAATAAGAACTACTTGGCTTGGTACCAGCAGAAA20810.12385555621024615No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT20500.12201051909226555No Hit
CTACCTTGGTCCCTCCGCCGAAAGTGGGAGTAGTATAAAATTGCTGACAGGAATAAACTGCCACATCTTCAGC20480.12189148443949259No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20410.12147486315478731No Hit
GGCTGAAGATGTGGCAGTTTATTCCTGTCAGCAATTTTATACTACTCCCA20170.12004644732151198No Hit
AGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20100.1196298260368067No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19860.11820141020353139No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT19730.11742768496050729No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT19630.11683251169664258No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19380.1153445785369808No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19200.11427326666202432No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19160.11403519735647843No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18880.11236871221765724No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18610.11076174440522253No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18500.11010705381497134No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18370.10933332857194723No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18370.10933332857194723No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCCACTG18070.10754780878035308No Hit
TCTCTACCTTGGTCCCTCCGCCGAAAGTGGGAGTAGTATAAAATTGCTGACAGGAATAAACTGCCACATCTTCAG18010.10719070482203426No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17990.10707167016926133No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG17710.10540518503044013No Hit
GTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTCTTTTATACAGGCCCAATAATAAGAACTA17690.10528615037766718No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17640.10498856374573484No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17430.10373869989161895No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17350.10326256128052717No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17190.10231028405834365No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCA17090.10171511079447894No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16960.10094138555145481No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA16870.10040572961397658No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC16840.10022717763481716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAT200.007237682651.9691145
GTGGTAT4650.051.5343361
TGGTATC4900.048.190612
GGTATCA13950.040.000461
ACCGTTC451.0458048E-438.0542458
GTATCAA28000.034.111261
CCGCTAT1353.7158425E-529.72542270
GTTAGCC704.070402E-529.3334143
TATCAAC32450.029.3183482
ATCAACG32900.028.7092973
GCGTTGT13050.028.56021362
ATTGTAC605.685836E-428.5185993
TCAACGC33400.028.4844444
TAGACGG13250.028.1535365
AACGCAG33900.028.0710016
CAACGCA33950.028.023825
AGTAGAC13350.027.9418163
GTAGACG13800.027.7746334
GTTGTCT13600.027.52926864
GTGCGAA500.00803419727.3868181
Produced by FastQC (version 0.11.5)