FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537021
Sequences flagged as poor quality0
Sequence length21-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25900.4822902642540981No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAAT24620.4584550697272546No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23210.43219911325627863No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20350.3789423504853628No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20180.37577673871226636No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20030.3729835518536519No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18140.3377893974351096No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17420.32438210051376015No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTC16880.3143266278227481No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAG15980.2975675066710613No Hit
GATTATTACTGCTGCTCAATTGCAGGAATTACCACTTGGGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT15920.2964502319276155No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGG15550.28956037100969984No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC15430.2873258215228082No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15420.2871396090655673No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14910.27764277374627805No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14370.267587301055266No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14350.26721487614078404No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13950.2597663778511455No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGT13590.25306272939047075No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13060.24319346915669965No Hit
GTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT13020.24244861932773576No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG12470.2322069341794827No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACA11760.21898584971537424No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11580.2156340254850369No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAA11480.21377190091262724No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11470.2135856884553863No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11370.21172356388297664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10850.2020405161064465No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10430.194219592902326No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC10240.19068155621474767No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9830.1830468454678681No Hit
CCCTGAGAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC9730.18118472089545845No Hit
GCCTGGAGCCCAGAGATTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC9270.1726189478623741No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9170.17075682328996444No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATGATGAGTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGA9130.1700119734610006No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8800.16386696237204879No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8800.16386696237204879No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTG8360.15567361425344634No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA8240.15343906476655475No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8150.15176315265138607No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8110.15101830282242223No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8070.15027345299345835No Hit
TTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGCCCT7960.14822511596380775No Hit
CTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC7820.14561814156243424No Hit
CAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGC7740.14412844190450652No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT7700.14338359207554266No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG7670.14282495470381978No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGG7600.14152146750313302No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGAATCTCTGGGCTCCAGG7580.1411490425886511No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7520.14003176784520532No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7390.13761100590107278No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7330.13649373115762697No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7290.1357488813286631No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7220.13444539412797638No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCAATTGCAGGAATTACCA7090.13202463218384383No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7010.1305349325259161No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6980.1299762951541932No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6880.12811417058178357No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6820.1269968958383378No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6810.12681068338109683No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT6770.12606583355213297No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC6640.12364507160800044No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG6600.12290022177903658No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT6600.12290022177903658No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC6560.12215537195007273No Hit
TCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT6490.12085188474938596No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT6370.1186173352624944No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG6350.11824491034801246No Hit
TCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACC6320.11768627297628956No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6300.11731384806180764No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6290.11712763560456667No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6290.11712763560456667No Hit
GTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAATC6290.11712763560456667No Hit
CATAAATGATGAGTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAATACCCAACATCA6270.11675521069008474No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC6250.11638278577560282No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6220.11582414840387992No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6130.11414823628871124No Hit
GGTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGGC6030.1122861117163016No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCAATTGCAGGAA6000.1117274743445787No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG6000.1117274743445787No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG5990.11154126188733773No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC5970.11116883697285582No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG5910.11005156222941002No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG5850.10893428748596423No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC5780.10763080028527748No Hit
GTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGGCA5740.10688595045631362No Hit
GACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGCC5690.1059548881701088No Hit
GTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC5630.104837613426663No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG5630.104837613426663No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC5490.1022306390252895No Hit
CAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG5480.10204442656804856No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5470.10185821411080759No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCAATT5470.10185821411080759No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG5430.10111336428184374No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTG5430.10111336428184374No Hit
GTCTGGCAACACGGCCTCCCTGAGAATCTCTGGGCTCCAGGCTGAGGACG5400.10055472691012084No Hit
GGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGT5380.10018230199563889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)