FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537021
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24610.4582688572700136No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAAT21630.4027775450122062No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20960.3903013103770616No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20770.38676327368948327No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17350.3230786133130734No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17210.32047163891169994No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT16250.3025952430165673No Hit
GATTATTACTGCTGCTCAATTGCAGGAATTACCACTTGGGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT15920.2964502319276155No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15830.29477431981244684No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15800.29421568244072394No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15110.2813670228910974No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG14920.27782898620351903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14680.27335988722973587No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14660.27298746231525395No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAG14290.2660976013973383No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGT14070.26200092733803704No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTC13840.2577180408214949No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13670.2545524290483985No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13480.2510143923608201No Hit
GTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT13460.25064196744633827No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGG13200.24580044355807315No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC12780.23797952035395264No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG12750.23742088298222974No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACA12250.2281102601201815No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAA11620.21637887531400077No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10750.20017839153403685No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10570.1968265673036995No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10420.194033380445085No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC10180.1895642814713019No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10010.18639866969820548No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10000.1862124572409645No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9760.18174335826718135No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9730.18118472089545845No Hit
CCCTGAGAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC9660.1798812336947717No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9450.17597077209271145No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9280.17280516031961507No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA9210.1715016731189283No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8860.16498423711549454No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8830.16442559974377166No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8470.15772195128309693No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTG8450.157349526368615No Hit
CTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC8320.15492876442448247No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8210.15288042739483185No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8200.1526942149375909No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATGATGAGTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGA8190.15250800248034993No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT7930.14766647859208484No Hit
GCCTGGAGCCCAGAGATTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC7820.14561814156243424No Hit
TTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGCCCT7540.14040419275968724No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7540.14040419275968724No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7520.14003176784520532No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7500.13965934293072338No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC7410.1379834308155547No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCAATTGCAGGAATTACCA7410.1379834308155547No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7400.13779721835831374No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7280.13556266887142218No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7260.13519024395694024No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7150.1331419069272896No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6910.12867280795350647No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGAATCTCTGGGCTCCAGG6860.12774174566730165No Hit
CAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGC6850.1275555332100607No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6800.12662447092385587No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGG6790.12643825846661488No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6750.12569340863765105No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG6560.12215537195007273No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6520.12141052212110885No Hit
TCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT6490.12085188474938596No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6290.11712763560456667No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6250.11638278577560282No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC6160.11470687366043414No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG6090.11340338645974739No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6060.1128447490880245No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT6060.1128447490880245No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCAATTGCAGGAA6020.11209989925906062No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG5960.11098262451561483No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG5910.11005156222941002No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC5910.11005156222941002No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA5870.10930671240044618No Hit
TCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACC5810.10818943765700038No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCAATT5760.10725837537079556No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG5730.10669973799907267No Hit
CAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG5700.10614110062734977No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG5690.1059548881701088No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT5600.10427897605494012No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5580.10390655114045819No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA5540.10316170131149435No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5510.10260306393977145No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTG5480.10204442656804856No Hit
GGTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGGC5420.10092715182460277No Hit
CATAAATGATGAGTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAATACCCAACATCA5390.10036851445287986No Hit
GTCTGGCAACACGGCCTCCCTGAGAATCTCTGGGCTCCAGGCTGAGGACG5390.10036851445287986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT100.008706865135.3577470
GGCCTAA300.002541117867.6788770
GTGGTAT2000.042.9216881
GGTATCA5650.042.5418551
GTATGAT350.00137883639.242691
TGGTATC2200.039.0124552
GGCTTAC451.02909304E-438.1526151
GTATCAA12500.037.3590431
TATCAAC14400.032.1852762
GTGTAAG553.3461777E-431.2157761
ATCAACG15100.030.6932433
GCTCGGT450.004634527530.64767337
AACGCAG15150.030.5919426
TATTCGC450.004717804530.5363124
CAACGCA15200.030.4913125
TCAACGC15200.030.4913124
ACGCAGA15900.029.1543547
AGAGTAC16000.028.97753711
CAGAGTA16000.028.97753710
TTATACT605.576384E-428.6091334
Produced by FastQC (version 0.11.5)