FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570402
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT43860.7689313852335721No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30470.5341846627466242No Hit
GTATAATAGTGTTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGTAATCCGTC27760.4866743103986311No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21520.3772777795309273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19990.3504545916739422No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19520.3422147888682017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16550.29014624773405423No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15990.28032861034849105No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAA15360.2692837682897325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15100.26472557950357817No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14870.26069333557736474No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13470.23614924211345684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13390.23474672248694778No Hit
ATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA13240.23211699818724338No Hit
ATTATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13110.22983790379416622No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13040.22861069912097082No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12480.21879306173540766No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12310.215812707529076No Hit
GATTACACTCTCACCATCACCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACACTATTATACTACC12110.2123064084628034No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11770.20634570005014008No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11750.20599507014351284No Hit
CTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTT11510.20178751126398575No Hit
GTGTAATCCGTCCCAGATCCACTGCCACCGAACCTGGATGGGACCCCACT11160.1956514878980088No Hit
GCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCGGTGGCAGTGGATCTGGGACGGATTACA10850.1902167243452863No Hit
GCATAGAGCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC10730.18811294490552277No Hit
GAGCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAG10710.1877623149988955No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10700.1875870000455819No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10500.18408070097930934No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10350.18145097667960491No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10140.17776936266001872No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10080.17671747294013695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9910.17373711873380526No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9840.1725099140606099No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT9800.17180865424735536No Hit
ATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCGGTGGCAGTGGATCTGGGACGGATTACACTCTCA9760.17110739443410086No Hit
AGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGCTCTATG9690.16988018976090546No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9660.16935424490096457No Hit
GGATTACACTCTCACCATCACCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACACTATTATACTAC9520.1668998355545738No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9310.1632182215349876No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9250.16216633181510584No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9070.15901066265546052No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9000.15778345798226515No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG8900.15603030844912885No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8740.15322526919611082No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT8700.1525240093828563No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGT8560.1500696000364655No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8540.14971897012983826No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8340.1462126710635657No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8230.14428420657711577No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8080.14165448227741137No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCC7820.13709629349125704No Hit
ACTTATTACTGTCAACACTATTATACTACCCCTCCGTGGACGTTCGGCCA7810.1369209785379434No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGAATTGCTA7750.13586908881806165No Hit
ATCCAGGTTCGGTGGCAGTGGATCTGGGACGGATTACACTCTCACCATCA7660.134291254238239No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7650.13411593928492538No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7550.13236278975178908No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAAC7470.13096027012528005No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7360.12903180563883015No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7340.1286811757322029No Hit
GAGTGTAATCCGTCCCAGATCCACTGCCACCGAACCTGGATGGGACCCCA7290.12780460096563476No Hit
ATGCAGCATAGAGCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGAATTGCTAATGCC7270.12745397105900752No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCTGTCAGGACACAGCATGGA7190.1260514514324985No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7150.125350191619244No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6950.12184389255297141No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6920.12131794769303053No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6920.12131794769303053No Hit
GTACCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA6850.12009074301983513No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6800.119214168253267No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC6800.119214168253267No Hit
CCCCTAAACTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCA6730.1179869635800716No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6670.11693507386018984No Hit
AACCTGGATGGGACCCCACTTTCCAATCTGGATGCAGCATAGAGCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG6510.11413003460717178No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6440.11290282993397639No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT6400.11220157012072186No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT6330.11097436544752648No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6320.11079905049421286No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC6290.11027310563427198No Hit
TCACCAGCCTGCAGCCTGAAGATTCTGCAACTTATTACTGTCAACACTAT6260.10974716077433108No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT6220.10904590096107658No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6180.10834464114782207No Hit
GGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGCTCTATGCTGCATC6160.1079940112411948No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6060.10624086170805853No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6000.10518897198817675No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5880.10308519254841322No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5840.10238393273515872No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCAT5830.10220861778184509No Hit
GTGATGGTGAGAGTGTAATCCGTCCCAGATCCACTGCCACCGAACCTGGA5770.10115672806196331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC200.006662488453.0641664
TCGTATG1150.045.15231340
ACGAGAC1600.045.10779622
CGTATGC1300.042.6353941
TCCGAGC1700.042.43578313
GTATCAA12250.042.367341
CGAGACA1900.041.60317223
TATGCCG1350.041.11444543
CGAGCCC1800.040.08175715
ATCTCCG1800.040.07824310
TCTCCGA1800.040.07824311
GCCGTCT1150.039.28120446
TATCAAC13350.038.8559532
CCCACGA1800.038.17979419
CCGAGCC1900.037.96885714
CTCGTAT1200.037.4816439
ATGCCGT1300.037.37534744
CTTATAC1950.037.0147671
TATACAC2250.036.6429633
ATCAACG14050.036.431063
Produced by FastQC (version 0.11.5)