FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698428
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45290.2666583452463101No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC40640.23928008723360658No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40280.23716048016165536No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36220.21325602262798307No Hit
GTGTATCTTGCATGCAGTAATAAACCCCAACATCCCCAGCCTCCACCGTGCTGATTTTCAGTGTGAAATCAGTGC34820.2050131062370616No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30950.18222733021358575No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29970.1764572887399407No Hit
CTATAAGGATCTCTGGCACAGAAATATACGGCCGTGTCCTCGAGTCTCAG29810.17551524115240683No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29600.17427880369376858No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29530.1738666578742225No Hit
CAAGATACACTGTTCACTTCCGGCACTGGGACCAAAGTGGATATCAAACG29440.17333675610623472No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA29190.171864806750713No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28740.16921529791077397No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28610.16844988424590268No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27450.16162003923628201No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26750.1574985810408213No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26390.15537897396887004No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25910.1525528312062684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25860.15225844133516406No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTAC25810.15196405146405972No Hit
GTGGAGGCTGGGGATGTTGGGGTTTATTACTGCATGCAAGATACACTGTT25610.15078649197964236No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25040.1474304474490529No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC24300.1430734773567087No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24040.14154265002696612No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23910.14077723636209483No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23070.13583148652754196No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22810.13430065919779938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21940.12917827544058388No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC21340.12564559698733183No Hit
TCCTTATAGTGGGAGCTATTATGACGACTACTGGGGCCGGGGAACCCTGG20980.12352598991538057No Hit
CGTATATAGTGATGGCAACACCTTCTTGAACTGGTTTCAGCAGAGGCCAG20880.1229372101731719No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGAG20320.11964004361680329No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20100.11834472818394422No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19590.11534195149867997No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19450.11451765985958781No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA18830.110867225457894No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18760.11045507963834793No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC18550.1092186421797097No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18530.10910088623126797No Hit
GATCAGGACTCCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC18090.10651025536554978No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAAC18020.10609810954600371No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG17770.10462616019048201No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17480.10291869893807686No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17360.10221216324742644No Hit
GGGCTGGAGTGGGTGGCCAACATAATTCAAGATGGAAGTGAAAAATACTA17220.10138787160833429No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17030.10026919009813781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT200.007291752451.87142634
GTATCAA20600.039.578511
GTGGTAT3750.035.9292371
ATCAACG24250.033.467043
TCAACGC24400.033.26134
TATCAAC24650.033.2041662
CAACGCA25100.032.4712835
AACGCAG25750.031.6516236
TGTTAGA7550.031.13658170
AGTGAGT15350.029.38773270
CGCAGAG28050.029.178568
AGAGTAC28150.029.07918711
CAGAGTA28850.028.2539110
AGTGCGT500.00769318727.63136310
TTCGGGG17300.027.54427170
GCAGAGT30350.026.9681289
CTACGGG950.00900613326.75176870
GGTATCA14100.026.4617941
ACGCAGA31250.026.1906767
TACGGGT2200.025.12016115
Produced by FastQC (version 0.11.5)