FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927513
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGA37930.40894305524558683No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGA35780.3857627871523095No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35390.38155799433538934No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGC31900.3439304893839763No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30360.32732694851716365No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGG29400.3169766892755142No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25600.2760069131106518No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24410.26317690425902385No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23580.2542282426230144No Hit
GTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG23070.24872966740088817No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22500.24258420097615885No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22060.23784033215706948No Hit
GTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC21630.233204278538414No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21240.22899948572149392No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20350.2194039328828814No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT20040.21606166166943214No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19770.2131506512577182No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19760.21304283605728438No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18820.20290820721650263No Hit
GTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG17510.1887844159596685No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17490.1885687855588008No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17160.18501088394448378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16980.18307021033667453No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16830.1814529823301668No Hit
GCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCC16720.18026701512539448No Hit
TCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTCAGGGACC16470.17757163511454827No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16010.17261213589459123No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15860.17099490788808352No Hit
GCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCC15720.16948549508200964No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15680.16905423428027422No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAG15190.16377128945901567No Hit
GTCAGGGACCCCTGACGGCCGATTGTTGTTACCATAGATGACGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA15010.1618306158512064No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14620.15762582303428632No Hit
GTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAG14540.15676330143081552No Hit
GTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCC14520.15654767102994782No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14400.15525388862474163No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT14390.1551460734243078No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG14330.15449918222170472No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14080.15180380221085849No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGAC13980.15072565020652No Hit
GTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13940.15029438940478462No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13880.1496474982021815No Hit
GCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCT13770.14846153099740922No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC13580.1464130421891661No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13570.14630522698873225No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG13380.14425673818048912No Hit
GGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAG13330.14371766217831988No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13330.14371766217831988No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGG13290.1432864013765845No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC13240.14274732537441523No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13230.14263951017398138No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGG13140.14166917337007676No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12980.13994413016313517No Hit
GATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGG12610.1359549677470828No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCT12610.1359549677470828No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12560.13541589174491353No Hit
GGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGG12430.13401429413927352No Hit
GTCTGGCGCCTCAGCCTCCCTGGCCGTCACTGGGCTTCAGGCTGAGGATG12340.1330439573353689No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGGACATCATAACCTGCCCCGATGTT12310.13272051173406735No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTA12230.13185799013059654No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12150.13099546852712576No Hit
CCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGG12020.12959387092148575No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11940.12873134931801494No Hit
GCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAG11790.1271141213115072No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11570.12474218690196257No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11510.12409529569935947No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11480.12377185009805794No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC11460.12355621969719023No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11400.12290932849458715No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA11350.1223702524924179No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11310.1219389916906825No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11230.12107647008721172No Hit
GTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTC10990.11848890527679935No Hit
CCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGC10980.1183810900763655No Hit
GCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCA10770.1161169708672547No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10700.11536226446421775No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10670.1150388188629162No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10670.1150388188629162No Hit
GGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGAC10660.11493100366248235No Hit
GTCCCTGACCGCTTCTCTGGCTCCCAGTCTGGCGCCTCAGCCTCCCTGGC10580.11406848205901157No Hit
GGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC10550.11374503645771002No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTC10510.11331377565597463No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10480.1129903300546731No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10460.11277469965380539No Hit
CTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGG10370.11180436284990077No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10340.11148091724859921No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG10220.11018713484339303No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10140.10932461323992224No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT10140.10932461323992224No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10100.10889335243818685No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9840.1060901572269068No Hit
TCACTGGGCTTCAGGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTAT9840.1060901572269068No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTC9680.10436511401996523No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGGACATCATAACCT9650.10404166841866368No Hit
AGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGG9620.10371822281736212No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9510.10253225561258979No Hit
GCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGGCTCCCAGT9410.10145410360825131No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9350.10080721240564822No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9300.10026813640347898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)