FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927513
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGA35870.38673312395621406No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35710.3850080807492725No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGA31920.344146119784844No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGC31550.3401569573687916No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28680.30921399484427714No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGG28540.3077045820382032No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25570.2756834675093503No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23480.25315009061867594No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22920.24711243939438046No Hit
GTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG22740.24517176578657118No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22560.24323109217876193No Hit
GTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC22230.23967319056444492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20890.22522595370630924No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20680.22296183449719842No Hit
GTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG20050.21616947686986598No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18850.20323165281780417No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18740.20204568561303185No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18430.19870341439958253No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT18100.19514551278526554No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17700.19083290476791162No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16960.1828545799358068No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16570.17864978711888677No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAG16330.1760622223084744No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16020.17271995109502508No Hit
GTCAGGGACCCCTGACGGCCGATTGTTGTTACCATAGATGACGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA15150.16334002865728028No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15150.16334002865728028No Hit
TCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTCAGGGACC15030.1620462462520741No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14900.16064464864643407No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14810.15967431184252945No Hit
GCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCC14740.1589196054394925No Hit
GTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14720.1587039750386248No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC14560.1569789318316832No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14530.1566554862303817No Hit
GCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCC14380.15503825822387396No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14360.15482262782300624No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14230.15342103021736622No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14050.15148035660955697No Hit
GTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCC13950.15040220460521847No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13910.14997094380348308No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT13900.14986312860304923No Hit
GTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAG13810.1488927917991446No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13680.14749119419350457No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA13660.14727556379263687No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC13440.1449036293830922No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG13390.14436455338092297No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG13300.14339421657701834No Hit
GGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAG13080.14102228216747364No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGGACATCATAACCTGCCCCGATGTT13070.1409144669670398No Hit
GCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCT13030.1404832061653044No Hit
GATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGG12940.13951286936139978No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12550.13530807654447968No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12450.1342299245401412No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGG12440.13412210933970736No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGAC12310.13272051173406735No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGG12300.1326126965336335No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12260.1321814357318981No Hit
GGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC12200.131534544529295No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12110.13056420772539037No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTC11810.12732975171237493No Hit
GTCTGGCGCCTCAGCCTCCCTGGCCGTCACTGGGCTTCAGGCTGAGGATG11710.12625159970803643No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA11420.12312495889545483No Hit
GTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTC11370.12258588289328559No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11230.12107647008721172No Hit
GCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAG11210.12086083968634402No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11160.12032176368417478No Hit
CCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGG11150.12021394848374092No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCT10980.1183810900763655No Hit
GGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGG10950.11805764447506395No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10930.11784201407419627No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10840.11687167727029164No Hit
GGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGAC10730.1156857100655193No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTA10690.1152544492637839No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC10660.11493100366248235No Hit
GTCCCTGACCGCTTCTCTGGCTCCCAGTCTGGCGCCTCAGCCTCCCTGGC10560.11385285165814388No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10530.11352940605684234No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10470.11288251485423924No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10410.11223562365163615No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10390.11201999325076845No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10360.11169654764946692No Hit
CCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGC10250.11051058044469458No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC10120.10910898283905456No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG10100.10889335243818685No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT10090.108785537237753No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9890.10662923322907604No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGGACATCATAACCT9820.1058745268260391No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9790.10555108122473754No Hit
GCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGGCTCCCAGT9760.10522763562343601No Hit
TCACTGGGCTTCAGGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTAT9720.10479637482170062No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9660.10414948361909752No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9610.10361040761692829No Hit
GCGCCAGACTGGGAGCCAGAGAAGCGGTCAGGGACCCCTGACGGCCGATT9600.10350259241649444No Hit
AGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGG9570.10317914681519288No Hit
GTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA9510.10253225561258979No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9500.10242444041215594No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9420.10156191880868516No Hit
GTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGC9370.10102284280651593No Hit
GGCCGTCACTGGGCTTCAGGCTGAGGATGAGGCTGATTATTCCTGCCAGT9370.10102284280651593No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9320.10048376680434669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC150.00223053869.9542157
TATGATA252.611721E-455.0070084
TATTCGC200.006837506352.720163
GTGGTAT3200.048.3512151
TGGTATC3350.046.181262
GGTATCA8850.042.736041
GTATCAA22000.040.3219871
TATCAAC24850.035.6936842
ATCAACG25400.035.056143
TCAACGC25700.034.5131534
AACGCAG26000.034.2489976
CAACGCA26050.034.0494425
CAGAGTA27800.032.3101410
CGCAGAG27800.032.060828
AGAGTAC28000.031.83353211
ACGCAGA29600.030.1030567
GCAGAGT29850.030.0911839
TTCGGGG3151.8189894E-1228.55519970
GAGTACG28250.028.50627712
AGTACGG28200.028.43478213
Produced by FastQC (version 0.11.5)