FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576323
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5910.10254666220157795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18200.041.030021
GGTATCA7500.040.3770751
TATCAAC21450.034.6499182
ATCAACG21700.034.567863
TCAACGC21650.034.488764
GTGGTAT3000.034.4122771
CAACGCA21950.034.1741455
AACGCAG22550.033.270636
ACGCAGA23250.032.2885487
CGCAGAG23850.031.4762578
TGGTATC3300.031.2811742
AGAGTAC24000.030.99525311
CAGAGTA24100.030.86664410
GCAGAGT25000.030.0309589
AGTACGG21150.028.82143213
GAGTACG22000.028.02096412
AATAGCG1757.2759576E-1226.0747262
GTACGGG23550.025.5895214
GTGTGCG951.0640448E-525.3762311
GACGTGT700.001205446925.07184662
Produced by FastQC (version 0.11.5)