FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218403
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5770.26419051020361445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4920.22527163088419114No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4380.2005466957871458No Hit
GTATTACTGTCATCAGAGTACTGATTTCCCGCTCACTTTCGGCGGAGGGA4310.19734161160789915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4300.19688374243943535No Hit
GTGTAAGCTCCTGCCAATGGTCTGACTGGCCCGGCAGGTGATGGTGACTT4060.18589488239630408No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA3730.17078519983699858No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3630.16620650815236054No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCTCCGCCGAAAGTG3560.16300142397311393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3550.1625435548046501No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT3420.15659125561462067No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3410.15613338644615687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3220.14743387224534463No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2970.13598714303374954No Hit
GATTTCACCCTCACCATCAATGGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTC2940.1346135355283581No Hit
GTCCTCCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGC2930.13415566635989432No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2930.13415566635989432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2860.1309505821806477No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2850.1304927130121839No Hit
CCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGCCTGGAAG2840.1300348438437201No Hit
GAGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCA2720.12454041382215446No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC2640.12087746047444405No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2460.11263581544209557No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2460.11263581544209557No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2420.11080433876824038No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2380.10897286209438516No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT2370.10851499292592134No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA2310.10576777791513853No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2290.10485203957821092No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2270.10393630124128331No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2250.1030205629043557No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC2190.1002733478935729No Hit
CCCCTGAGGAGGACTGCGAAGCATACTTGATGAGGAGCTTTGGAGACTGA2190.1002733478935729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGT200.00703738552.3235948
TAGGCCT200.007328127551.7907939
TAAACAA200.00752457951.4455230
ACAATAT200.00762771651.2687268
CAGTCGT400.009765631548.1471670
TGGTATC1500.040.9868162
GTATCAA10700.040.5584871
GGTATCA4150.040.3468631
ACTTCGT350.00127439539.86559348
GTGGTAT1750.035.1476481
ATCAACG12450.034.5671923
GTATTAG400.002716298234.1713261
TCAACGC12650.033.750674
CAACGCA12750.033.485965
AACGCAG13050.032.7236566
TATCAAC13850.031.3196472
GTACGAA450.004457841630.88381643
CGGGACT450.004824947630.38843217
AGGTATC450.00484662530.3606032
CGCAGAG14350.030.0109628
Produced by FastQC (version 0.11.5)