FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262095
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.041.9840431
GGTATCA12850.040.8024441
ATCAACG38750.036.4609073
CAACGCA39250.036.172035
TCAACGC39000.036.1388244
AACGCAG40450.035.098946
TATCAAC41200.034.376362
ACGCAGA42650.033.0447437
CAGAGTA45100.031.48009310
CGCAGAG45050.031.3608048
AGAGTAC45250.031.29958511
TGGTATC5900.030.9555972
GTGGTAT6250.030.9067761
GCAGAGT48350.029.3628969
AGTACGG39400.028.68751513
GAGTACG39900.028.32802212
CTCGTAT1004.8812944E-727.68757439
TAGACTA906.9693397E-626.8022275
ACGTTAC658.714389E-426.50874716
TACGGGG19600.025.84500315
Produced by FastQC (version 0.11.5)