FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262095
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12670.1003886395239661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.041.1498261
GTATCAA33650.041.115361
GTGGTAT4850.036.190251
TCAACGC39250.034.806664
ATCAACG39500.034.5863653
CAACGCA39950.034.282925
AACGCAG41500.033.0063976
TATCAAC41800.032.683292
ACGCAGA44100.030.9129317
CGCAGAG45450.029.9225398
TGGTATC5900.029.7460772
CAGAGTA45900.029.55651510
AGAGTAC46350.029.27071811
GCAGAGT50600.026.878139
AGTACGG41000.025.2795613
GAGTACG41450.025.08818812
TACGGGC8500.024.30535715
GTACGGG45750.022.42911314
TACGGGA15250.022.12717215
ACGGGAT3950.021.78932216
Produced by FastQC (version 0.11.5)