FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018308
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTACTCCTATCCCACAC36660.36000895603294875No Hit
CCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG34410.3379134800080133No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTA31520.3095330685804295No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31360.3079618347297674No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26960.26475290383656025No Hit
GGATTACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26080.25611111765791883No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24960.24511248070328429No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTC24000.23568507759931182No Hit
CTCCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC23110.22694508930500398No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22100.21702667562269962No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21710.2131967931117108No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGTAACAACATTGGAAGT20890.20514421962706764No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGT19750.19394917844110035No Hit
GGATAGGAGTAGTGATCTTATAATTTTCGGCGGAGGGACCAAGATGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19320.189726487467446No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19220.1887444663107822No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18770.1843253711057951No Hit
GTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG18650.18314694571779855No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18270.1794152653224761No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17950.17627279762115194No Hit
CCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCT17760.17440695742349072No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16990.16684539451717947No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT16250.15957843795786736No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16200.15908742737953546No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA16100.15810540622287167No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16090.15800720410720528No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16010.15722158718187426No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15640.1535881089022182No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15220.14946362004423022No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGC15000.14730317349956987No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCAC14880.1461247481115733No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG14770.14504452483924313No Hit
GGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC14420.14160745079091985No Hit
GTATATTACTGTGCGAAACAGACGGACGTGGGAGATTACAATTTTTGGAGTGGTTATCCGGGGCACTTGGATTA14170.13915239789926034No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14080.1382685788582629No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13970.13718835558593276No Hit
GGGTGGCCGTGTTCCCAGAATTGGAGCCAGACAATCGCTCAGGGATCCCTGAGGGCCGTTTACTATCCTCATAGA13870.13620633442926894No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13640.13394768576894223No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13610.13365307942194307No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13570.13326027095927753No Hit
GCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCA13540.1329656646122784No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13530.13286746249661202No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13510.13267105826527925No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13370.13129622864594995No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAATTGGAGCCAGA13230.12992139902662064No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12730.12501129324330162No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12660.12432387843363697No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC12650.1242256763179706No Hit
GTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCG12450.122261634004643No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12410.12186882554197749No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12370.12147601707931195No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11980.11764613456832314No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11840.11627130494899383No Hit
TCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCGATTGTCTGGCTCCAATTCTGGGAACACGGCCACC11790.11578029437066192No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11760.11548568802366278No Hit
CTACGCAGACTCCGTGAAGGGCCAATTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTGCAAATGAA11740.11528928379233001No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGG11710.11499467744533087No Hit
ACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTAC11630.11420906051999985No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11440.11234322032233862No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATC11420.11214681609100588No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT11400.11195041185967311No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11350.1114594012813412No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11220.11018277377767827No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11210.1100845716620119No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG11180.10978996531501276No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11140.10939715685234722No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11070.10870974204268258No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT10940.10743311453901964No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC10860.1066474976136886No Hit
CTATTACTGTCAGGTGTGGGATAGGAGTAGTGATCTTATAATTTTCGGCG10760.10566547645702477No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCA10710.10517446587869289No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10690.10497806164736012No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT10680.10487985953169375No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10470.10281761510269978No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10430.10242480664003424No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10350.10163918971470322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT200.00736348251.74165334
GTATCAA15450.044.799071
GGTATCA5950.039.9331471
GTGGTAT2000.039.600371
TATCAAC17550.039.209532
TTATACA1950.038.817432
ATCAACG18250.037.7055973
CAACGCA18650.037.0813835
TCAACGC18650.037.0813834
CTTATAC2100.036.074871
CGTATGC1850.035.53693841
AACGCAG19500.035.465026
AGGGGGG2800.034.87246770
TATACAC2200.034.4063573
TCGTATG1950.033.69289840
CGCAGAG21400.032.3146678
AGAGTAC21550.031.9300911
TGGTATC2300.031.41452
ATGCCGT2150.030.6403344
CAGAGTA22550.030.5141210
Produced by FastQC (version 0.11.5)