FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887480
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15000.16901789336097714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26950.039.638641
GGTATCA11350.036.9216541
ATCAACG30950.034.62283
TCAACGC31100.034.4558074
AACGCAG31400.034.13436
TATCAAC31350.034.0714842
CAACGCA31400.034.017235
ACGCAGA33400.032.1029937
AGAGTAC33300.031.38439411
CTCGTTA550.004946443330.2437169
CAGAGTA35050.030.0135810
CGCAGAG35950.029.6413678
GCAGAGT36500.029.1038199
TGGTATC5100.028.9578632
AGTACGG30250.028.29817213
GAGTACG30250.028.07087712
GTGGTAT5850.026.4224931
TACGGGC7350.025.25895115
GTACGGG33850.025.08549514
TACGGGG14150.024.53995715
Produced by FastQC (version 0.11.5)