FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789939
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.043.589971
GGTATCA8850.043.2664151
ATCGTAC350.001239408740.10518362
TATCAAC26850.037.356172
TCAACGC27000.037.273944
ATCAACG27100.037.13643
CAACGCA27250.037.0584565
AACGCAG28200.035.8100366
CGAATTA400.002608564734.4656315
ACGCAGA29750.034.2918557
CGCAGAG30600.033.1140378
AGAGTAC30800.032.45140511
CAGAGTA31250.032.09439510
GCAGAGT33000.030.7057449
GTGGTAT3050.030.5376191
AGTACGG29500.027.572513
AGTACTT6900.027.472613
GAGTACG30100.027.02288212
TACGGGC7800.025.62828815
GTACGGG32600.024.95057714
Produced by FastQC (version 0.11.5)