FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064502
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39070.18924660765647117No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTAATTACTGGAGTTGGATCCG38610.18701846740763634No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37130.17984966834616772No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT34060.1649792540767701No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33270.16115266538855375No Hit
GTATTATGGTAGCTCACCGGGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT31570.15291823403416416No Hit
GTATTATTGTCAGTATTATGGTAGCTCACCGGGGACCTTCGGCCAAGGGA29840.14453848918528536No Hit
GTAATAAACGGCCGTGTCCGCGGCCGTCACAGAGGTCAAGTGCAGGGAGACCAGGTTCTTAGACGTGTCTAGTGA29160.14124471664352953No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27450.13296184745764353No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26480.12826337780249183No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26410.12792431298201698No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25290.12249927585441912No Hit
CTCCTGCAGGGTCAGTCAGAGTGTTGGTAGCAACTACTTAGGCTGGTACC25090.12153051922449096No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24320.11780080619926744No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23650.114555471489008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23240.11256952039765522No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGAT23080.11179451509371267No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATT22830.11058356930630243No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22680.1098570018338563No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21860.10588509965115073No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21780.10549759699917946No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21560.10443196470625847No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21440.10385071072830156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)