FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817493
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTA200.00709866452.222247
CGACGTT200.007186013352.0609442
GGTATCA9900.044.820521
GTATCAA28700.044.1050451
TATCAAC33350.037.620722
TCAACGC33750.037.1748434
ATCAACG33850.037.065023
GTCGGAT851.4061606E-436.1800170
CAACGCA35050.035.7960325
AACGCAG36350.034.610416
GTCGTAT400.002556051134.60897439
ACGCAGA38600.032.5929647
CGCAGAG38750.032.378098
AGAGTAC39500.031.76331311
TCGTATG553.1746528E-431.49956140
CAGAGTA40350.031.094210
CGTTCGT450.004449212530.9062845
ACGGGAT1850.029.7343216
GCAGAGT42250.029.6958799
AGTACGG27300.029.5893413
Produced by FastQC (version 0.11.5)