FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433905
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26170.18250860412649372No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23380.16305124816497607No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGTACAGCTTCAGGGAGA21370.1490335831174311No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21320.14868488498192No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21190.14777826982959122No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19430.13550409545960157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18060.12594976654659829No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16420.1145124677018352No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15990.11151366373644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15890.11081626746541787No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15830.11039782970280458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15540.10837538051684037No Hit
TCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTCCTGTCAACAGTTT14470.10091324041690349No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14350.10007636489167693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24850.037.505341
TATCAAC27650.033.3188552
GTGGTAT3250.032.925631
ATCAACG28700.032.340323
TCAACGC28850.032.0525784
CAACGCA29150.031.6043365
AACGCAG30050.030.6577846
TAATGAT9750.029.02426770
GGTATCA17700.027.8880751
ACGCAGA35100.026.539967
GCAGAGT35550.026.3010659
CGCAGAG35550.026.2040148
GTGTATA1759.094947E-1225.63091513
AGTACGG34300.023.94096613
GAGTACG35200.023.32883812
GTACTAG750.00198695623.0125371
CGAATCG750.001991394223.00370821
TGTTAGA8100.021.73834270
AGAGTAC42850.021.66009111
CAGAGTA43850.021.16613210
Produced by FastQC (version 0.11.5)