FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433905
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24820.17309375446769487No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22420.15635624396316353No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGTACAGCTTCAGGGAGA21990.15335743999776832No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18920.13194737447738866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17480.12190486817466988No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17070.1190455434634791No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17050.11890606420927466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17020.11869684532796804No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16140.11255975814297321No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16120.11242027888876878No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16060.1120018411261555No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15130.1055160558056496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.037.391941
GTATCAA22700.036.584481
TATCAAC25850.031.9830672
ATCAACG26400.030.9252953
TATATCG450.004636404630.64861123
TCAACGC26600.030.5632674
CAACGCA27300.029.6534085
AACGCAG28000.028.7910526
GTACTAG605.4604997E-428.7160341
TAATGAT10950.026.83352770
CGCAGAG31600.025.5235188
CGAACTA1501.0495569E-925.28510529
ACGCAGA32150.025.191457
GCAGAGT32400.024.999699
TGTTAGA7950.024.6395970
GGTATCA17100.024.5849571
TCGGTTA750.00188442423.22313154
AGTACGG30100.022.78758813
GAGTACG31050.022.20139112
GTATACA1103.2975844E-521.9286081
Produced by FastQC (version 0.11.5)