FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216135
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46410.38161881698989014No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA45390.3732315902428595No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43280.3558815427563552No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGGCCCTTTT41090.33787367356420134No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT37350.30712050882508934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34440.2831922442820904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33510.27554506695391545No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33130.27242041385208055No Hit
GTCTCCAGCCACGCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTTGGGCCAGTGAGGATATTAGCAG32000.26312868225978203No Hit
GTGTAGAACCGAGGCCAGTTGCTACGCCGCTGGCAGTAATAAACTGCAAA31890.26222417741451404No Hit
GTTCTACACTTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTGTGG31190.25646823749008124No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30010.24676536733175183No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29810.24512081306762817No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29720.24438076364877254No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29390.24166724911296855No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28950.23804922973189654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27020.22217928108310345No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26990.2219325979434849No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCAGCGGCGTAGCAAC25590.2104207180946194No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTTGGGCCAGTGAGG25580.21033849038141322No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23700.19487968029865105No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23360.19208393804964086No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA23320.19175502719681614No Hit
GTGCAGCCACAGTTCGTTTGATGTCGAGCTTGGTCCCCTGGCCAAAAGTG23190.19068606692513576No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCAGCGGCGTAGCAACTGG22630.18608131498558958No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGTTGATACCAGGCTAAGTAGCTGCTA22570.1855879487063525No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22510.1850945824271154No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21980.18073651362718776No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA21150.17391161343107467No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21130.1737471580046623No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20950.17226705916695104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19940.16396206013312667No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAATTTGTGTTGACACAGTC19740.16231750586900304No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGTTGATACCAGGC19620.16133077331052886No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGTTGATACCAGGCTAAGTAGCTGCTAATATCCTCA19380.1593573081935805No Hit
CTCCTGTTGGGCCAGTGAGGATATTAGCAGCTACTTAGCCTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCT19350.15911062505396192No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19340.15902839734075574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19030.15647933823136412No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18790.15450587311441577No Hit
GTAATAAACGGCCGTGTCCGCGGCGGTCATAGAGTTCACCTTCAGAGAGAACTGGTTCTCGGACTTGTCTACTGA18760.1542591899747972No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18280.15031225974090048No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCGAGCTTGGTCCCCTGGCCAAAAGTGTAGAACCGAGG18090.14874993318998303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17920.14735206206547793No Hit
GCGTAGCAACTGGCCTCGGTTCTACACTTTTGGCCAGGGGACCAAGCTCG17650.14513191380891102No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17290.14217171613348847No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17260.14192503299386994No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17060.14028047872974628No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC16960.13945820159768446No Hit
TCTATGATGCATCCAAAAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16660.136991370201499No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15780.12975533143935503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15720.1292619651601179No Hit
CATCTATGATGCATCCAAAAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15630.1285219157412623No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAAAAGGGCCGCTGGCATCCCA15450.127041816903551No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15430.12687736147713863No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15400.1266306783375201No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15340.12613731205828302No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15200.12498612407339646No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15170.12473944093377791No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15090.12408161922812845No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC14870.12227260953759245No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14800.1216970155451492No Hit
GTTTATTACTGCCAGCGGCGTAGCAACTGGCCTCGGTTCTACACTTTTGGCCAGGGGACCAAGCTCGACATCAAA14640.12038137213385028No Hit
GGATATTAGCAGCTACTTAGCCTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC14580.11988800585461318No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14330.11783231302445864No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGG14270.11733894674522154No Hit
CAACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGCGTGGCTGG14240.11709226360560299No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14120.1161055310471288No Hit
GCTAATATCCTCACTGGCCCAACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGCGTGGCTGGAGA14000.11511879848865464No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13990.11503657077544846No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13610.11191191767361353No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13500.11100741282834554No Hit
CTTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTGTGGCTGCACCA13390.11010290798307754No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13250.10895171999819099No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13130.1079649874397168No Hit
CACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGCCAGCGGCGTAGCAACTGGCCTCGGTTCTA13050.10730716573406736No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13030.10714271030765499No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12730.10467587891146952No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12710.10451142348505717No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12630.10385360177940771No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12580.1034424632133768No Hit
CTCTTACTTTGACCACTGGGGCCAGGGGACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT12520.10294909693413971No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG12510.10286686922093354No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12510.10286686922093354No Hit
GAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12420.10212681980207788No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12290.10105785953039752No Hit
GTGTTGACACAGTCTCCAGCCACGCTGTCTTTGTCTCCAGGGGAAAGAGC12270.10089340410398516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATC150.00229983169.41798450
GTGGTAT3050.049.6334341
GTGTATA754.119829E-836.6986581
ACGGACT400.002619030234.43897625
CGAGCTA400.002620087534.43614224
CAACGCA21500.034.4007455
TCAACGC21600.034.4007454
ATCAACG21650.034.32133
AACGCAG21850.033.6991926
ATAATGG6000.031.68013670
AGAGTAC23300.031.46341711
CGCAGAG23550.031.129418
TGTTAGA11850.030.93539670
TCGCTAT450.004664875630.60990724
CTACGCT450.004688402630.5784424
GCAGAGT24100.030.5618029
ACGTGAT900.00500394430.17155870
GTATAAG1601.8189894E-1227.9540541
GTCCTTA500.00783987827.5239941
TGTATAA1005.1460665E-727.52062
Produced by FastQC (version 0.11.5)