FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257504
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATGATGTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT50050.39801066239153116No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42020.33415400666717565No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39970.31785187164414586No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38790.3084682036796702No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36530.2904960938494033No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31070.24707674886123623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29550.2349893121612337No Hit
GTATTACTGTCAACAGTATGATGATGTCCCGTGGACGTTCGGCCAAGGGA28620.22759370944346896No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27760.22075476499478333No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26240.20866732829478077No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25840.20548642390004326No Hit
TCATCATACTGTTGACAGTAATACGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAAT25700.20437310736188516No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25260.20087411252767387No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG24440.19435325851846197No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24220.19260376110135632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23760.1889457210474082No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22500.17892587220398504No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATAACTAC22330.1775739878362216No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21940.17447260605135254No Hit
GATCTACGCTGCTTCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGATTTTA21730.17280263124411532No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21400.17017838511845687No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21300.1693831590197725No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20730.16485037025727153No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT20100.15984044583555998No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20010.159124742346744No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19940.15856808407766496No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19630.1561028831717434No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19620.15602336056187494No Hit
GTACTATGTGGGCTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG18780.14934346133292617No Hit
GCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT18580.14775300913555742No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18540.1474349186960837No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18450.14671921520726774No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18230.1449697177901621No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18150.14433353691121462No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18100.1439359238618724No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18040.14345878820266178No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17880.14218642644476676No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17860.1420273812250299No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17670.14051645163752957No Hit
GATCAGCACTGAGCACAGAAGACTCACCATGGAGTTGGGGCTGAGCTGGG17400.13836934117108177No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17180.13661984375397612No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17170.1365403211441077No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17070.13574509504542331No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17000.13518843677634426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16870.13415464284805456No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG16830.1338365524085808No Hit
CAGTAATACGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCTCAGATCCACTGCCA16830.1338365524085808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16780.13343893935923862No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16690.13272323587042267No Hit
ATGATGTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA16470.13097373845331706No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16090.12795187927831642No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16090.12795187927831642No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGAGACA15990.12715665317963204No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15960.12691808535002672No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTCTCTACAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTA15600.12405527139476295No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15350.12206720614805203No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15180.12071532178028856No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAAGACTCACCATGGAGTTGGGGCTGAG15080.11992009568160418No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15030.119522482632262No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14900.11848868870397232No Hit
GTATATTACTGTGTCAGAGGGGTAATTCGGGGAGTTTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTC14820.1178525078250248No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGCGTCAGGACATTGACAA14720.11705728172634042No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14660.11658014606712981No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14550.11570539735857699No Hit
GGGCTGGAGTGGGTGGCCAATATAAAGCAAGATGGAAGTGAGAAGTACTA14420.11467160343028729No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14390.11443303560068198No Hit
CTCCTGTGCAGTCTCTGGTTTCACCTTTAGTAGTTATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG14310.11379685472173448No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14250.11331971906252386No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14110.11220640252436573No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14090.11204735730462885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14080.11196783469476042No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14070.11188831208489199No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14060.11180878947502353No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13990.11125213120594447No Hit
ACTGTCAACAGTATGATGATGTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG13970.11109308598620761No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13900.11053642771712854No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13750.10934358856910197No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA13680.1087869303000229No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT13670.10870740769015448No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA13650.10854836247041759No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGAAGCAGCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13190.10489032241646945No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT13100.1041746189276535No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA13070.1039360510980482No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC13010.10345891543883756No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12920.10274321195002163No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12920.10274321195002163No Hit
ACGTATTACTGTCAACAGTATGATGATGTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12730.10123228236252131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)