FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828852
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10000.12064880099221574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20950.038.0831951
GGTATCA8900.035.935391
TATCAAC23050.034.3048362
ATCAACG22950.034.3045123
TCAACGC23200.034.083044
CAACGCA23400.033.644815
AACGCAG24550.032.354716
ACGCAGA26350.030.0212737
AGAGTAC26250.029.74974611
CGCAGAG26850.029.5974588
TGGTATC3950.029.5924052
GTGGTAT4000.029.2313161
CAGAGTA27350.028.42744410
GCAGAGT29500.027.0553199
GAGTACG22900.024.63741312
AGTACGG23400.024.40500813
CGTCTAT851.4672615E-424.29273228
GTACGGG25450.022.30400714
CCAATAC1253.6092533E-622.0028233
CCGTCTA953.1176044E-421.7342927
Produced by FastQC (version 0.11.5)