FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302623
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45120.3463780387725382No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41950.3220425249669321No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36520.2803574019497583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33250.2552542063206315No Hit
GTCTACATCCGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA33140.25440975631475876No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32880.25241378357360494No Hit
GATCTATGGTGCATCCAGTTTGCTAAGTGCGGCCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA30420.23352881071499582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29120.22354894700922678No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27810.21349231512110567No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25150.1930719786154551No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23950.18385979673320677No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23740.1822476649038133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23440.1799446194332512No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22590.17341932393332532No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22250.17080920573335495No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22040.16919707390396146No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21400.164283910233429No Hit
ACTTACTACTGTCAACAGAGTTTCAGTCTCCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21070.16175056021581072No Hit
GTGCGGCCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT21010.1612899511216983No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20710.1589869056511362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20420.15676062836292617No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19960.15322929197473098No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCAGAGGGAGA19620.1506191737747606No Hit
CAGCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTCCCTCTGACGTT19580.15031210104535234No Hit
CCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT19540.15000502831594406No Hit
GCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG19290.14808582375714233No Hit
GATTTCACTCTCAGCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTC19220.14754844648067783No Hit
GTCTCAGTCAGGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGG18930.14532216919246782No Hit
CTGTTGATGCTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT18610.14286558735720156No Hit
GTCTCCATCCTCCCTGTCTACATCCGTAGGAGACAGAGTCACCATCACTT18340.14079284643369572No Hit
GCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18330.14071607825134363No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18240.140025164610175No Hit
GTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAGCTCCTGA17260.13250188273967217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16740.12850993725736456No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGCTGAGAGTGAAATCTGTCCCAGATCCACTGCCA16300.1251321372338735No Hit
GGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16250.12474829632211316No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATTGAGATGACCCAGTC16220.12451799177505694No Hit
GTCAGGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15560.11945129173982034No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCAGCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTA15420.11837653718689137No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15080.11576641898692101No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14990.11507550534575238No Hit
GTTGATGCTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG14980.11499873716340032No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14950.1147684326163441No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14680.1126956916928382No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGGCCGCA14660.11254215532813408No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14540.11162093713990924No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14310.10985526894581166No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14260.10947142803405129No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14220.10916435530464302No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14150.10862697802817853No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTCCCTCTGACGTTCGGCCAAGGGA14040.10778252802230576No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13890.10663100528702472No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13340.1024087552576609No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13340.1024087552576609No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13240.10164107343414018No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13200.1013340007047319No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13180.10118046434002778No Hit
CCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG13180.10118046434002778No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13090.10048955069885915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29250.042.918971
TATCAAC33100.037.805552
ATCAACG35500.035.3465233
TCAACGC35350.035.3020024
CAACGCA35950.034.9040765
AACGCAG37250.033.7782366
ACGCAGA39500.031.7659077
CGCAGAG40900.030.7649778
AGAGTAC40600.030.7394511
TATACAC2600.030.4114173
GACGCGT2300.029.92503426
CGAGACG2450.029.48620423
TCGTATG2400.028.8299340
ACGCGTA2400.028.6836727
GCAGAGT44100.028.53269
CGTATGC2450.028.26121341
CGCGTAG2500.027.54055828
AGACGCG2500.027.52997625
CAGAGTA45850.027.51960810
CGTCGTT658.737915E-426.49658230
Produced by FastQC (version 0.11.5)