FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888580
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT69860.7861982038758468No Hit
CTTCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTC65080.7324045105674222No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52780.5939814085394675No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC38510.433388102365572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37140.41797024466001936No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34660.3900605460397488No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33710.37936933084246777No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCG33410.3759931576222737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32500.3657520988543519No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31130.3503342411487992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30590.34425712935244995No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30380.34189380809831416No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTGAGA30060.33829255666344055No Hit
GTTGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTGAGAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA28940.3256881766413829No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28420.31983614305971325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27750.3122960228679466No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG27700.31173332733124764No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27080.30475590267617997No Hit
GAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAGAAGTAAT26890.30261765963672377No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26760.30115465124130636No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26400.2971032433770735No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24250.27290733529901645No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23990.2699813185081816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22650.25490107812464835No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22560.2538882261585901No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22250.2503995138310563No Hit
GGCCAAAAGTGTACGGATTACTTCTATACTGTTGGCAGTAATAAGTTGCA22100.2487114272209593No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21410.24094622881451305No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC21020.23655720362826083No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20580.23160548290530958No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA20350.22901708343649418No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTGAGAGTCTTAGTAG20050.22564091021630017No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19920.22417790182088274No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19810.22293997164014495No Hit
GAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA19470.21911364199059172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18240.20527133178779625No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA17820.20054468927952462No Hit
GATTACTTCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATT17750.19975691552814603No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17570.19773121159602963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17270.19435503837583562No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17250.19412996016115602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17200.193567264624457No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17170.1932296473024376No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16900.19019109140426296No Hit
GGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT16880.18996601318958337No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16770.18872808300884558No Hit
GTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCT16770.18872808300884558No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16730.1882779265794864No Hit
GCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT16210.18242589299781675No Hit
GTGAGAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGG15870.17859956334826352No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15650.17612370298678792No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15650.17612370298678792No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAAGACTCTCA15540.17488577280605008No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15440.1737603817326521No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15140.17038420851245809No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14950.16824596547300188No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTG14950.16824596547300188No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTG14750.16599518332620586No Hit
GCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA14470.16284408832069144No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14090.15856760224177902No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14040.15800490670508No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13920.1566544374170024No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTGGAGA13850.15586666366562382No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC13630.1533908033041482No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13370.15046478651331338No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13350.15023970829863378No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGC12790.14393751828760495No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12770.14371244007292536No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12740.14337482275090593No Hit
GAGTCACCATCGCTTGCCGGGCCAGTGAGAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAG12700.14292466632154674No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12530.14101150149677014No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12520.1408989623894303No Hit
CTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCGG12230.13763532827657612No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12190.1371851718472169No Hit
CACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC12030.1353845461297801No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11830.1331337639829841No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11690.1315582164802269No Hit
GGTTTCTGCTGATACCAGGCCAACCAGCTACTAAGACTCTCACTGGCCCG11670.13133313826554727No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11600.13054536451416868No Hit
CAGTATAGAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11580.13032028629948905No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11550.12998266897746968No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11540.12987012987012986No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC11400.12829458236737265No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11230.12638141754259605No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11080.12469333093249906No Hit
GTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCT10960.12334286164442144No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10950.12323032253708163No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10820.12176731414166424No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10750.12097954039028563No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10750.12097954039028563No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC10730.12075446217560604No Hit
GTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGC10720.12064192306826622No Hit
ACTTATTACTGCCAACAGTATAGAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10660.11996668842422743No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10540.11861621913614982No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10460.1177159062774314No Hit
GTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCG10260.11546512413063541No Hit
ATCTGGGACAGAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATT10040.11298926376915978No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG9920.11163879448108217No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9870.11107609894438317No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9800.11028832519300456No Hit
GGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGG9600.10803754304620856No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9560.10758738661684934No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9540.10736230840216975No Hit
GATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGCCAACC9530.10724976929482996No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTT9460.10646199554345136No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9350.10522406536271353No Hit
GTGTACGGATTACTTCTATACTGTTGGCAGTAATAAGTTGCAAAATCATC9320.10488644804069415No Hit
TTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGC9210.10364851785995632No Hit
GAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGT9190.10342343964527673No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC9170.10319836143059713No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9130.10274820500123794No Hit
TCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC9110.10252312678655834No Hit
GTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGT9080.10218550946453893No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGCC9030.10162281392783992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA451.9842233E-458.14657270
GTGGTAT2150.051.2496381
GTATCAA18900.044.818011
CCCACGA602.9710645E-740.14285319
CGGTCAT350.001253302840.0149260
TATCAAC21700.039.162842
ACTACGC557.511513E-637.54885527
ATCAACG22800.037.1225053
TCAACGC22850.036.89074
TATACAC853.0486262E-936.430123
CAACGCA23300.036.3258865
AACGCAG23650.035.9337736
TCGGGAC501.891084E-434.4062233
ACGCAGA25150.033.7906047
CGCAGAG25450.033.5293668
AGAGTAC25250.033.38613511
ATGTGTA1056.6211214E-1032.76967211
CAGAGTA26100.032.69434410
TTATACA959.027644E-932.5953672
GCAGAGT26350.032.5147329
Produced by FastQC (version 0.11.5)