FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888580
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTC75670.851583425240271No Hit
GTATAGAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT67850.7635778433005469No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54370.6118751266064958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33410.3759931576222737No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32260.36305116027819667No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31750.35731166580386686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31690.356636431159828No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31610.3557361183011096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31600.3556235791937698No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG30080.33851763487812014No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28990.3262508721780819No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCG26570.29901640820185016No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26380.29687816516239396No Hit
GTTGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTGAGAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA26280.2957527740889959No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26240.2953026176596367No Hit
GAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAGAAGTAAT26030.2929392964055009No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTGAGA25960.2921515226541223No Hit
GGCCAAAAGTGTACGGATTACTTCTATACTGTTGGCAGTAATAAGTTGCA25240.28404870692565665No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24130.2715568660109388No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23830.2681806927907448No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23210.2612032681356772No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22060.2482612707916001No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21960.24713587971820208No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21760.24488509757140606No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21240.23903306398973642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20570.2314929437979698No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20440.23002993540255237No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20340.22890454432915436No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20040.22552837110896037No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA19070.21461207769699972No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19000.2138243039456211No Hit
GATTACTTCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATT18910.2128114519795629No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18790.2114609826914853No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18520.20842242679331066No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18260.20549641000247587No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18130.20403340160705846No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17750.19975691552814603No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17350.195255351234554No Hit
GAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16900.19019109140426296No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16500.18568952711067097No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTGAGAGTCTTAGTAG16430.18490175335929238No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16390.18445159692993315No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16340.18388890139323413No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA16180.18208827567579733No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16040.18051272817304012No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15980.17983749352900133No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15490.17432307726935112No Hit
GGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT15100.16993405208309886No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14970.16847104368768148No Hit
GCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT14910.16779580904364266No Hit
GTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCT14860.16723311350694364No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14630.16464471403812825No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAAGACTCTCA14500.16318170564271084No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14060.15822998491975962No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13970.1572171329537014No Hit
GTGAGAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGG13900.1564293592023228No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13510.1520403340160706No Hit
GGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC13460.15147763847937157No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13400.15080240383533278No Hit
GCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA13320.14990209097661436No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACT12950.14573814400504176No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTG12880.14495037025366314No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTG12870.14483783114632334No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT12800.14405005739494472No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12380.1393234148866731No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC12260.1379729455985955No Hit
GAGTCACCATCGCTTGCCGGGCCAGTGAGAGTCTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGCAAG11990.1349343897004209No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG11960.13459677237840148No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11930.13425915505638208No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC11760.13234599023160548No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11710.1317832946949065No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11700.13167075558756666No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGC11680.13144567737288707No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11620.13077044272884827No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11590.13043282540682888No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11490.12930743433343087No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11480.12919489522609107No Hit
GGTTTCTGCTGATACCAGGCCAACCAGCTACTAAGACTCTCACTGGCCCG11360.12784442593801346No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11250.12660649575727564No Hit
GTGTACGGATTACTTCTATACTGTTGGCAGTAATAAGTTGCAAAATCATC11230.12638141754259605No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11060.12446825271781943No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTGGAGA10980.12356793985910104No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC10940.12311778342974185No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10940.12311778342974185No Hit
CACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC10890.12255508789304284No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10870.12233000967836324No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10580.11906637556550902No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10520.1183911409214702No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10510.11827860181413041No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10460.1177159062774314No Hit
CAGTATAGAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10460.1177159062774314No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10440.11749082806275181No Hit
GTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGC10400.11704067163339262No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10380.11681559341871299No Hit
CTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCGCTTGCCGG10110.11377703752053839No Hit
TCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC10040.11298926376915978No Hit
ACTTATTACTGCCAACAGTATAGAAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9990.11242656823246078No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9840.11073848162236376No Hit
GGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGG9720.10938801233428617No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9570.10769992572418915No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9540.10736230840216975No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9540.10736230840216975No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG9520.10713723018749015No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC9470.10657453465079114No Hit
ATCTGGGACAGAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATT9420.10601183911409214No Hit
GTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCT9390.10567422179207275No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9280.10443629161133493No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9260.10421121339665533No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9250.10409867428931555No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGGCAGCC9100.10241058767921853No Hit
TTATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAGGC9070.10207297035719912No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGATTACTTCTATACTGTT9010.10139773571316033No Hit
GTATCAGCAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATAAGGCGT8930.10049742285444191No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8890.10004726642508271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG252.6166157E-454.985911
TCGTATA200.006921205752.55797261
TCGACTT200.007447503551.59284219
CGTAACG200.007457460751.57541312
GTTATTA805.6994722E-542.17444670
GTGGTAT2450.042.0810471
GTATCAA17500.040.8466761
TATCAAC19550.036.381592
TACAGCG400.00250166934.76138350
ATCAACG20600.034.527193
TCAACGC20600.034.527194
CAACGCA20850.034.1131945
AACGCAG21100.033.8756686
CGCAGAG22600.031.791868
AGAGTAC23000.031.39372811
ACGCAGA23050.031.0203047
GTATAAA450.004710831730.5477261
GTTATAT450.004710831730.5477261
TAATACA450.004714723730.5425684
CAGAGTA24000.030.37218510
Produced by FastQC (version 0.11.5)