FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358173
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59420.4374994938052811No Hit
GTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCGAGGTGGAAATGAAACGAA57210.42122763447660944No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA50300.37035046345347755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36290.26719718327488473No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA36090.2657246168198013No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35790.26351576713717617No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGA35700.26285311223238866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33240.2447405448348627No Hit
GTGTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCA31540.23222372996665372No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31410.23126656177084953No Hit
CTCCTGCAGGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCT29540.21749806541581962No Hit
CTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCGAGGTGGAAATGAAACGAACTGTGGCTGCACCAT29400.21646726889726123No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT28570.21035611810866509No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC27840.20498125054761065No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCTAGTGAGAGTATTAGCAG27100.19953275466380202No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26940.19835470149973533No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26920.19820744485422698No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTCGGTCCCTTGGCCGAACGTCTTCGGAGGCCAGTTAGTAGTCTC26660.19629310846261852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26330.1938633738117309No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25760.1896665594147432No Hit
GTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCTAGTGAGA24910.1834081519806387No Hit
GTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA24350.17928496590640514No Hit
CAATAATACAGAGCAGTGTCCTCACTTCTCAGACTGCTCATCTGAAGAAA24010.17678160293276335No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23430.17251116021302146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23330.17177487698547975No Hit
CATCTATGGTGCATCCACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTG23210.1708913371124297No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22950.16897700072082128No Hit
GTATTATTGCGTGAAAGACCTCGGGTACGGCTCCCTCCTTCACTATTGGGGCCAGGGAACCCTGGTCACCGTCTC22160.1631603632232418No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT22140.16301310657773346No Hit
GAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGACTACTAACTGG21220.15623930088434979No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTCGGTCC20340.1497600084819828No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20230.1489500969316869No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20190.14865558364067022No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19980.14710938886283265No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19520.1437224860161408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19270.14188177794728654No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGC19160.14107186639699065No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT19110.14070372478321982No Hit
CCACTGGAGAGATAGAGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTC19020.14004106987843226No Hit
GTGCCACCCTCTCCTGCAGGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGG18940.1394520432963989No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18750.13805310516406966No Hit
CACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGTCTCACCATCAGCGG18210.13407717573534447No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17950.13216283934373604No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17300.12737699836471494No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17050.1255362902958607No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17000.12516814868208984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16860.12413735216353144No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16820.12384283887251478No Hit
CTCCTGTTCAGCCTCTGGCTTCAGTTTCAGAAACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA16740.1232538122904814No Hit
GGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA16720.12310655564497307No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16610.12229664409467719No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16580.12207575912641469No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16360.12045593602582293No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16260.11971965279828121No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16090.11846797131146033No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16010.11787894472942695No Hit
GAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC15760.11603823666057271No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAGATAGAGATGACGCAGTC15620.1150074401420143No Hit
TCTATGGTGCATCCACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT15600.11486018349650598No Hit
CTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTTCCATTCAGT15540.11441841355998095No Hit
GATAGAGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAA15470.11390301530070175No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15290.11257770549112668No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15170.11169416561807664No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14980.1102952274857474No Hit
CTTCTCAGACTGCTCATCTGAAGAAACAGCGTGTCCTTGGAATTGTCTCTGGAGATGGTGAATCTGCCCCTCA14780.108822661030664No Hit
GTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA14740.10852814773964731No Hit
AGTTAGTAGTCTCCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACT14510.1068346963163014No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14490.10668743967079304No Hit
GGATACCAGCGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGG14490.10668743967079304No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC13900.10234336862829699No Hit
GGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCT13690.10079717385045939No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13670.10064991720495106No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13660.10057628888219688No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13640.10042903223668855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)