FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358173
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58930.4338917059903268No Hit
GTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCGAGGTGGAAATGAAACGAA56530.41622090852932575No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA46270.34067824938354685No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA34510.2540913418246424No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34320.25269240369231316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34200.2518088638192631No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGA34170.2515879788510006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32870.24201629689295842No Hit
GTGTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCA31890.2348007212630497No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30680.22589169420979505No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT29410.21654089722001543No Hit
CTCCTGCAGGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCT27540.20277240086498555No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC27360.20144709105541048No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27310.20107894944163962No Hit
CTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCGAGGTGGAAATGAAACGAACTGTGGCTGCACCAT26240.19320071890694338No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTCGGTCCCTTGGCCGAACGTCTTCGGAGGCCAGTTAGTAGTCTC25470.18753133805487224No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25350.1866477981818222No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24970.1838499219171637No Hit
GTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA24620.18127293062076777No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCTAGTGAGAGTATTAGCAG24220.17832779771060095No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24050.17707611622378003No Hit
CATCTATGGTGCATCCACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTG23090.17000779723937967No Hit
GTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCTAGTGAGA23080.1699341689166255No Hit
CAATAATACAGAGCAGTGTCCTCACTTCTCAGACTGCTCATCTGAAGAAA22570.1661791244561628No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22190.16338124819150432No Hit
GTATTATTGCGTGAAAGACCTCGGGTACGGCTCCCTCCTTCACTATTGGGGCCAGGGAACCCTGGTCACCGTCTC22170.163233991545996No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT21940.16154054012265007No Hit
GAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGACTACTAACTGG21320.1569755841118915No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20310.14953912351372026No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20200.1487292119634244No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19850.14615222066702843No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19600.14431151259817415No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19520.1437224860161408No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18920.13930478665089058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18510.13628602541796958No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT18270.13451894567186948No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTCGGTCC18190.13392991908983612No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17690.1302485029521276No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17530.12907044978806087No Hit
CCACTGGAGAGATAGAGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTC17430.1283341665605192No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17290.12730337004196077No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17170.12641983016891073No Hit
GTGCCACCCTCTCCTGCAGGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGG17090.12583080358687737No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGC16940.12472637874556482No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAGATAGAGATGACGCAGTC16690.12288567067671055No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16310.12008779441205206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16120.11868885627972284No Hit
CACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGTCTCACCATCAGCGG16110.11861522795696866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16070.11832071466595197No Hit
GGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA15950.11743717479290193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15780.11618549330608104No Hit
CTCCTGTTCAGCCTCTGGCTTCAGTTTCAGAAACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA15780.11618549330608104No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15350.1130194754276517No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15110.11125239568155161No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15060.11088425406778077No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15020.11058974077676409No Hit
GATAGAGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAA14940.1100007141947307No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14810.1090435459989265No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14720.10838089109413897No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14540.10705558128456391No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14510.1068346963163014No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14430.10624566973426802No Hit
GAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC14390.10595115644325134No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14270.10506761657020128No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13850.10197522701452613No Hit
CTTCTCAGACTGCTCATCTGAAGAAACAGCGTGTCCTTGGAATTGTCTCTGGAGATGGTGAATCTGCCCCTCA13830.10182797036901779No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13780.10145982875524695No Hit
TCTATGGTGCATCCACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT13760.1013125721097386No Hit
GGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCT13680.10072354552770524No Hit
AGTTAGTAGTCTCCTGACAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACT13630.10035540391393437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)