FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161161
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCCTCAGGCTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTC37470.3226942689256701No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24450.2105651154318824No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21610.18610683617517296No Hit
GCATAATAGTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20080.1729303688291288No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16650.14339096817753955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16090.13856820888748417No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15400.13262588047652307No Hit
GATGTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCA14950.12875044890415716No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14370.1237554482108855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14340.12349708610606108No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13820.11901880962243824No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13470.11600458506615362No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13330.11479889524363977No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13000.11195691209057142No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12690.10928717034071934No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12650.10894268753428679No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGGGCATTAGGAATGATTTAGGCTGGTATCAGCAGACA12400.10678966999408351No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12290.10584234227639405No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12250.10549785946996153No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12250.10549785946996153No Hit
GCATACATCAGGCGCTTAGGGGCTTCCCCTGGTGTCTGCTGATACCAGCC12250.10549785946996153No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT11810.10170854859920372No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11750.10119182438955493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.042.537981
GTGGTAT3900.042.3852231
ATCAACG40200.035.1862533
TATCAAC40150.035.058642
TCAACGC40800.034.6688084
TCGTATG1003.6925485E-1034.60050240
CAACGCA41450.034.208185
AACGCAG42200.033.6817676
AGAGTAC44950.031.77701411
CGCAGAG45150.031.6348888
CAGAGTA45500.031.39289510
ACGCAGA46450.030.6741057
GCAGAGT46500.030.6424399
TAAACCG605.5003614E-428.6797945
CTCGTAA500.00756327927.72721743
AGTACGG41850.027.71590813
GAGTACG42200.027.56759812
TATACAC1503.6379788E-1127.5326023
GGTATCA23550.025.4446331
GTGTATA2200.025.03179213
Produced by FastQC (version 0.11.5)