FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342632
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16690.48711153657568473No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16040.46814074575638004No Hit
CTACAAACTCCTCGGACTTTTGGCGAGGGGACCAAGCTGGAGATCAAACG10320.30119778654649887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9940.29010717037521305No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9800.28602115389105515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9490.27697354596184826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9330.27230381283709637No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8500.24807957225244578No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTCGCCAAAAGTC8270.2413668308856149No Hit
GTAATGGATATAACTATTTGGATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACATCTCCTGATCTATTTGGGTT7770.22677391487076512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7540.22006117350393425No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA7490.21860188190244928No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT7380.21539144037918231No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7180.20955427397324244No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6990.2040089658875995No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG6890.2010903826846296No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA6810.1987555161222536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6740.19671250788017464No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAGAGCCTCCTG6580.19204277475542272No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGCGAGGGGACCAAGCTGGAGATCAAACGAA6540.19087534147423474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6460.1885404749118588No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6440.18795675827126482No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6290.18357888346680987No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTACAAACT6260.1827033085059189No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC6150.17949286698265193No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG5870.1713208340143361No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5540.16168950944453525No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGCGAGGGGA5490.16023021784305028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5300.15468490975740737No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5100.14884774335146747No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT5090.14855588503117045No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5040.1470965934296855No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5010.1462210184687945No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4960.14476172686730954No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4910.14330243526582456No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA4880.14242686030493357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4880.14242686030493357No Hit
CTCTACAAACTCCTCGGACTTTTGGCGAGGGGACCAAGCTGGAGATCAAA4760.1389245604613696No Hit
GGATATAACTATTTGGATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAAT4610.1345466856569147No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4480.13075252749305377No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4430.1292932358915688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4410.1287095192509748No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGCGAGGGGA4400.1284176609306778No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT4330.12637465268859885No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA4330.12637465268859885No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTACAAAC4270.12462350276681688No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT4190.1222886362044409No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4160.12141306124354993No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4100.11966191132176797No Hit
AGCCAGGCCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAATCGGGCC4060.11849447804057997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4060.11849447804057997No Hit
GTCTCCACATCTCCTGATCTATTTGGGTTCTAATCGGGCCCCCGGGGTCC3990.11645146979850102No Hit
GTCAGGGACCCCGGGGGCCCGATTAGAACCCAAATAGATCAGGAGATGTGGAGACTGGCCTGGCTTCTGCAGGTA3960.11557589483761004No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3800.11090616171285811No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3800.11090616171285811No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA3750.10944687011137315No Hit
CTCCTGCATAGTAATGGATATAACTATTTGGATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACATCTCCTGAT3730.10886315347077913No Hit
GTACCTGCAGAAGCCAGGCCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAATCGGGCCCCCGGGGTCCCTGA3720.10857129515048215No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3590.10477713698662122No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3520.10273412874454224No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3480.10156669546335427No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3480.10156669546335427No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG3430.1001074038618693No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCCA3430.1001074038618693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)